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ELISA Troubleshooting Low Signal
A number of problems commonly arise during ELSIA that can result in low signal and poor color development. Here are some troubleshooting tips to help you improve your experiment and get better data.
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ELISA Troubleshooting Saturated Signal
If your ELISA signal is too high, the results of the experiment can become unusable. Saturated signal can cause wells to appear uniformly reactive, or cause the standard curve to become unusable. Before you repeat your ELISA experiment, read these troubleshooting tips to identify possible sources of your saturated signal error, and solutions to solve it.
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ELISA Troubleshooting High Background
The signal-to-noise ratio of your ELISA assay is critical for the proper interpretation of results. High background signal can ruin the data of an entire assay. Before you redo your experiment, consult this guide for tips to help resolve the source of your high background results.
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ELISA Troubleshooting Well-to-Well Variation
High well-to-well variation can be a symptom of a flawed experiment, or it can be a simple error that’s easy to fix. Errors in plate handling or loading, or mistakes in the usage of reagents are all common causes of well variance in ELISA. Use this troubleshooting guide to help solve some of the common sources of error that drive high well-to-well variability.
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ELISA Troubleshooting Low Sensitivity
When using small amounts of precious protein sample, the sensitivity of your ELISA assay can be its most important feature. Boster pre-coated ELISA kits are guaranteed to have consistently high sensitivity, but other ELISA formats can encounter errors that reduce their sensitivity. Use this troubleshooting guide to avoid common sources of reduced ELISA sensitivity:
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ELISA Troubleshooting Matrix Effect
Matrix Effect occurs when the target antigen interacts with matrix components in plasma or serum samples. These matrix components can be endogenous biological components such as phospholipids, carbohydrates, and metabolites. Matrix components can reduce the binding of the antibody to the target protein, or non-specifically bind the antibody, generating weak or noisy results. Here are some tips to reduce matrix effect:
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