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ELISA Troubleshooting: Low Sensitivity
When using small amounts of precious protein sample, the sensitivity of your ELISA assay can be its most important feature. Boster pre-coated ELISA kits are guaranteed to have consistently high sensitivity, but other ELISA formats can encounter errors that reduce their sensitivity. Use this troubleshooting guide to avoid common sources of reduced ELISA sensitivity:
|Incorrect assay type||Assay format inherently not sensitive enough||Switch to a more sensitive detection system|
|Switch to a more sensitive assay type|
|Use Boster high-sensitivity ELISA kits|
|Incompatible sample type||Use sample types (e.g. serum vs. cell extract) verified to work as positive control|
|Include a positive control in your experiment|
|Reagent/sample problems||Insufficient target||Reduce dilution factor or concentrate sample|
|Inactive substrate||Run a control to verify reporter enzyme reacts with substrate|
|Insufficient substrate||Increase concentration of substrate|
|Mixing or substituting reagents from different kits||Avoid mixing components from different kits|
|Plate problems||Improper storage of ELISA kit||Follow manufacturer storage recommendations|
|Poor target adsorption to wells||Covalently link target to wells|
Keywords: ELISA, Troubleshooting, Low Sensitivity, Low Detection LimitClick for more troubleshooting tips