GDNF Glial-Derived Neurotrophic Factor Human Recombinant Protein

GDNF protein, Human

Glial derived Neurotrophic Factor Human Recombinant produced in E. coli is a non-glycosylated disulfide-linked homodimer containing 2 x 135 amino acids and having a total molecular mass of approximately 30kDa. GDNF is purified by proprietary chromatographic techniques.

Product Info Summary

SKU: PROTP39905
Size: 2ug, 10ug, 1mg
Origin Species: Human
Source: Escherichia coli

Product Name

GDNF Glial-Derived Neurotrophic Factor Human Recombinant Protein

View all GDNF recombinant proteins

SKU/Catalog Number

PROTP39905

Size

2ug, 10ug, 1mg

Description

Glial derived Neurotrophic Factor Human Recombinant produced in E. coli is a non-glycosylated disulfide-linked homodimer containing 2 x 135 amino acids and having a total molecular mass of approximately 30kDa. GDNF is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

GDNF Glial-Derived Neurotrophic Factor Human Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP39905)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

GDNF was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

23.72kDa

Reconstitution

It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Amino Acid Sequence

MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Biological Activity

The ED50 was determined by the proliferation of rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of greater than 1.0x107 units/mg.

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Reconstitution

It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For GDNF (Source: Uniprot.org, NCBI)

Gene Name

GDNF

Full Name

Glial cell line-derived neurotrophic factor

Weight

23.72kDa

Superfamily

TGF-beta family

Alternative Names

Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3 GDNF ATF, ATF1, ATF2, HFB1-GDNF, HSCR3 glial cell derived neurotrophic factor glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on GDNF, check out the GDNF Infographic

GDNF infographic

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Size

Total: $250

SKU:PROTP39905

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