HER2 (Phospho-Tyr877) Colorimetric Cell-Based ELISA Kit

The HER2 (Phospho-Tyr877) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HER2 (Phospho-Tyr877) protein expression profile in cells. The kit can be used for measuring the relative amounts of HER2 (Phospho-Tyr877) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HER2 (Phospho-Tyr877).

Product Info Summary

SKU: EKC2428
Size: 1 kit, containing 2 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

HER2 (Phospho-Tyr877) Colorimetric Cell-Based ELISA Kit

See all ErbB2/Her2 products

SKU/Catalog Number

EKC2428

Size

1 kit, containing 2 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The HER2 (Phospho-Tyr877) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HER2 (Phospho-Tyr877) protein expression profile in cells. The kit can be used for measuring the relative amounts of HER2 (Phospho-Tyr877) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HER2 (Phospho-Tyr877).

Storage & Handling

4°C/6 Months

Cite This Product

HER2 (Phospho-Tyr877) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2428)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC2428 is reactive to ERBB2 in Human, Mouse, Rat samples

Application Guarantee

EKC2428 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-ERBB2 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-ERBB2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For ERBB2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04626

Gene ID

2064

Gene Name

ERBB2

Full Name

Receptor tyrosine-protein kinase erbB-2

Weight

137.91kDa

Superfamily

protein kinase superfamily

Alternative Names

CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomade ERBB2 CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1 erb-b2 receptor tyrosine kinase 2 receptor tyrosine-protein kinase erbB-2|c-erb B2/neu protein|herstatin|human epidermal growth factor receptor 2|metastatic lymph node gene 19 protein|neuro/glioblastoma derived oncogene homolog|neuroblastoma/glioblastoma derived oncogene homolog|proto-oncogene Neu|proto-oncogene c-ErbB-2|tyrosine kinase-type cell surface receptor HER2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2|v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2|v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ERBB2, check out the ERBB2 Infographic

ERBB2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ERBB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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