Show Order Info
SKU BA1050
Pack size 0.5 ml
Applications Western blot
Price: $95.00

Product Overview

Product Name Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate
Synonyms HRP-conjugated goat anti-mouse IgG
Description Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through Dot Blot, WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex.
Reagent Type Secondary antibody, reporter enzyme labeled
Label HRP (Horseradish Peroxidase)
Host Rabbit
Target Species Mouse
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule mouse IgG
Preparation Affinity purified from rabbit antiserum
Specificyity Mouse IgG specific; No cross-reactivity with related species
Form Supplied Liquid: concentrated buffered stock solution
Formulation 0.5 mg HRP-conjugated secondary antibody
0.01 M PBS (pH 7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application ELISA*, WB*, Dot blot
*Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage 4C for 1 year
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Protein detection/quantification
Technique Immunodetection of target antibody with HRP reporter enzyme
Equipment Needed WB/Dot blot/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
Compatibility with Reagents Incompatible with sodium azide and metals
incompatible with high phosphate concentrations

Main Advantages

Specific High signal-to-noise ratio
Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast Generates strong signals in a relatively short time span
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate Images

Click the images to enlarge.

Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate
Write a review for BA1050

Publications

Li, M., Wang, P., Zheng, Z., Hu, K., Zhang, M., Guan, X.,…, & Liu, Y. (2017). Japanese encephalitis virus counteracts BST2 restriction via its envelope protein E. Virology, 510, 67-75. Advance online publication. doi: 10.1016/j.virol.2017.07.008
Liao, Z., Wang, J., Tan, H., & Wei, L. (2017). Cinnamon extracts exert intrapancreatic cytoprotection against streptozotocin in vivo. Gene, 627, 519-523. doi: 10.1016/j.gene.2017.07.014
Chen, X., Zhang, Q., Bai, J., Zhao, Y., Wang, X., Wang, H., & Jiang, P. (2017). The nucleocapsid protein and non-structural protein 10 of highly pathogenic porcine reproductive and respiratory syndrome virus enhance CD83 production via NF-κB and Sp1 signaling pathways. Journal of Virology. Advance online publication. doi: 10.1128/JVI.00986-17
Chen, J., Wang, Q., Zhou, J., Deng, W., Yu, Q., Cao, X.,…, & Xu, X. Porphyra polysaccharide-derived carbon dots for non-viral co-delivery of different gene combinations and neuronal differentiation of ectodermal mesenchymal stem cells. Nanoscale. doi: 10.1039/C7NR03327C
Fang, X., Li, S., Han, Q., Zhao, Y., Gao, J., Yan, J., & Luo, A. (2017). Overexpression cdc42 attenuates isoflurane-induced neurotoxicity in developmental brain of rats. Biochemical and Biophysical Research Communications. Advance online publication. doi: 10.1016/j.bbrc.2017.06.108
Wu, H., Zhou, J., Ou, W., Li, Y., Liu, M., & Yang, C. (2017). TAK1 as the mediator in the protective effect of propofol on renal interstitial fibrosis induced by ischemia/reperfusion injury. European Journal of Pharmacology. Advance online publication. doi: 10.1016/j.ejphar.2017.06.009
Zhao, J., Lv, K., Li, Z.H., Wu, J., Gao, W., Wong, T.S.,…, & Lei, W.B.. (2017). Functional significance of the long non-coding RNA RP11-169D4.1 as a metastasis suppressor in laryngeal squamous cell carcinoma by regulating CDH1. Oncology Reports, 38(1), 211-220. Advance online publication. doi: 10.3892/or.2017.5645
Jin, D., Yu, X., Chen, B., Li, Z., Ding, J., Zhao, X., & Qi, G. (2017). Combined immunotherapy of breast cancer with EGF and VEGF vaccines from DNA shuffling in a mouse model. Immunotherapy. doi: 10.2217/imt-2017-0004
Wang, L., Zhou, Z., Chen, Y., Yuan, S., Du, Y., Ju, X.,…Wang, X. (2017). The alpha 7 nicotinic acetylcholine receptor of deciduous dental pulp stem cells regulatesosteoclastogenesis during physiological root resorption. Stem Cells and Development. Advance online publication. doi: 10.1089/scd.2017.0033
Wu, P., Jiang, X., Sang, Q., Annan, E., Cheng, T., & Guo, X. (2017). Inhibition of miR-274-3p increases BmCPV replication by regulating the expression of BmCPVNS5 gene in Boombyx mori. Virus Genes. Advance online publication. doi: 10.1007/s11262-017-1466-7.
Ma, L., Su. L., Liu, H., Zhao, F., Zhou, D., & Duan, D. (2017). Norovirus contamination and the glycosphingolipid biosynthesis pathway in Pacific oyster: A transcriptomics study. Fish & Shellfish Immunology, 66, 26-34. Advance online publication. doi: 10.1016/j.fsi.2017.04.023.
Gang Wang,1 Yue Jing,2 Lingsen Cao,3 Changchang Gong,1 Zhunan Gong,1,3 and Xiangrong Cao3 Onco Targets Ther. 2017; 10: 55–66. Published online 2016 Dec 20. doi: 10.2147/OTT.S121619 A novel synthetic Asiatic acid derivative induces apoptosis and inhibits proliferation and mobility of gastric cancer cells by suppressing STAT3 signaling pathway
Xiao Chenga, b, , 1, , Zijun Houa, b, d, 1, , Jingbo Suna, b, , Yan Huanga, b, , Lixin Wanga, b, , Ziyi Zhoua, b, , Li-Hua Zhouc, , Yefeng Caia, b, Protective effects of Tongxinluo on cerebral ischemia/reperfusion injury related to Connexin 43/Calpain II/Bax/Caspase-3 pathway in rat ☆

FAQs

Q: Is this product suitable for IHC? Keyword: application, Immunohistochemistry
A: Please beware that this product is not suitable for IHC. Small package of secondaries (0.5 ml) can do 15-75 slides and 1ml can do 30-150 slides. The difference is in the way customer uses the reagent.