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SKU BA1054
Pack size 0.5 ml
Applications Western blot
Price: $95.00

Product Overview

Product Name Goat Anti-Rabbit IgG Secondary Antibody, HRP Conjugate
Synonyms HRP-conjugated goat anti-rabbit IgG
Description Goat Anti-Rabbit IgG Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through Dot Blot, WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex.
Reagent Type Secondary antibody, reporter enzyme labeled
Label HRP (Horseradish Peroxidase)
Host Goat
Target Species Rabbit
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule rabbit IgG
Purification Immunoaffinity Chromatography
Specificity Rabbit IgG specific; No cross-reactivity with related species
Form Supplied Liquid: concentrated buffered stock solution
Formulation 0.5 mg HRP-conjugated secondary antibody
0.01 M PBS (PH 7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application ELISA*, WB*, Dot blot
*Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage 4C for 1 year
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Protein detection/quantification
Technique Immunodetection of target antibody with HRP reporter enzyme
Equipment Needed WB/Dot blot/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
Compatibility with Reagents Incompatible with sodium azide and metals
incompatible with high phosphate concentrations

Main Advantages

Specific High signal-to-noise ratio
Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast Generates strong signals in a relatively short time span
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

Goat Anti-Rabbit IgG Secondary Antibody, HRP Conjugate Images

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Goat Anti-Rabbit IgG Secondary Antibody, HRP Conjugate
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Publications

Xiong, Q., Ru, Q., Chen, L., Tian, X., & Li, C. (2017). Mitochondrial dysfunction and inflammatory response in the cytotoxicity of NR8383 macrophages induced by fine particulate matter. Environmental Toxicology and Pharmacology, 55, 1-7. Advance online publication. doi: 10.1016/j.etap.2017.07.017
Xie, L., Liu, S., Cheng, J., Wang, L., Liu, J., & Gong, J. (2017). Exogenous administration of mitochondrial DNA promotes ischemia reperfusion injury via TLR9-p38 MAPK pathway. Regulatory Toxicology and Pharmacology, 89, 148-154. doi: 10.1016/j.yrtph.2017.07.028
Su, H., Zhu, G., Rong, X., Zhou, Y., Jiang, P., & Chen, P. (2017). Upregulation of ATG4A promotes osteosarcoma cell epithelial-to-mesenchymal transition through the Notch signaling pathway. International Journal of Clinical and Experimental Pathology, 10(7), 7975-7982. Retrieved from http://www.ijcep.com/files/ijcep0044902.pdf
Qi, X., Qin, L., Du, R., Chen, Y., Lei, M., Deng, M., & Wang, J. (2017). Lipopolysaccharide Upregulated Intestinal Epithelial Cell Expression of Fn14 and Activation of Fn14 Signaling Amplify Intestinal TLR4-Mediated Inflammation. Frontiers in Cellular and Infection Microbiology, 7, 315. doi: 10.3389/fcimb.2017.00315
Wang, W., Peng, Y., Yang, H., Bu, H., Guo, G., Liu, D.,..., & Gao, F. (2017). Potential role of CXCL10/CXCR3 signaling in the development of morphine tolerance in periaqueductal gray. Neuropeptides. Advance online publication. doi: 10.1016/j.npep.2017.07.004
Liu, L., Gao, H., Wang, H., Zhang, Y., Xu, W., Lin, S.,..., & Guo, J. (2017). Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA‑200 and the downregulation of PI3K‑Akt signaling pathway. Oncology Letters, 14(3), 3741-3747. doi: 10.3892/ol.2017.6580
Hou, J., Ma, T., Cao, H., Chen, Y., Wang, C., Chen, X.,..., & Han, X. (2017). TNF-α-induced NF-κB activation promotes myofibroblast differentiation of LR-MSCs and exacerbates bleomycin-induced pulmonary fibrosis. Journal of Cellular Physiology. Advance online publication. doi: 10.1002/jcp.26112
Wang, P.F., Li, Z.G., Zhang, Y., Ju, X.H., Liu, X.W., Zhou, A.M., & Chen, J. (2017). NLRP6 Inflammasome Ameliorates Brain Injury after Intracerebral Hemorrhage. Frontiers in Cellular Neuroscience, 11, 206. doi: 10.3389/fncel.2017.00206
Gong, J.H., Gong, J.P., & Zheng, K.W. (2017). Checking transfer efficiency and equal loading via qualitative optical way in western blotting. Electrophoresis. Advance online publication. doi: 10.1002/elps.201700266
Guo, X., Guo, N., Zhao, J., & Cai, Y. (2017). Active targeting co-delivery system based on hollow mesoporous silica nanoparticles for antitumor therapy in ovarian cancer stem-like cells. Oncology Reports, 38(3), 1442-1450. doi: 10.3892/or.2017.5829
Zhang, X.D., Dong, X.Q., Xu, J.L., Chen, S.C., & Sun, Z. (2017). Hypoxia promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells via inducing Twist1 expression. European Review for Medical and Pharmacological Sciences, 21(13), 3061-3068. Retrieved from http://www.europeanreview.org/article/13074
Li, M., Wang, P., Zheng, Z., Hu, K., Zhang, M., Guan, X.,…, & Liu, Y. (2017). Japanese encephalitis virus counteracts BST2 restriction via its envelope protein E. Virology, 510, 67-75. Advance online publication. doi: 10.1016/j.virol.2017.07.008
Yan, P., Chen, K., Wang, Q., Yang, D., Li, D., & Yang, Y. (2017). UCP-2 is involved in angiotensin-II-induced abdominal aortic aneurysm in apolipoprotein E-knockout mice. PLoS One, 12(7), e0179743. doi: 10.1371/journal.pone.0179743
Xu, Y., Zheng, Y., Liu, H., & Li, T. (2017). Modulation of IGF2BP1 by long non-coding RNA HCG11 suppresses apoptosis of hepatocellular carcinoma cells via MAPK signaling transduction. International Journal of Oncology, 51(3), 791-800. doi: 10.3892/ijo.2017.4066
Liao, Z., Wang, J., Tan, H., & Wei, L. (2017). Cinnamon extracts exert intrapancreatic cytoprotection against streptozotocin in vivo. Gene, 627, 519-523. doi: 10.1016/j.gene.2017.07.014
Zhao, P., Sui, B.D., Liu, N., Lv, Y.J., Zheng, C.X., Lu, Y.B.,…, & Jin, Y. (2017). Anti-aging pharmacology in cutaneous wound healing: effects of metformin, resveratrol, and rapamycin by local application. Aging Cell. Advance online publication. doi: 10.1111/acel.12635
Chen, J., Wang, Q., Zhou, J., Deng, W., Yu, Q., Cao, X.,…, & Xu, X. Porphyra polysaccharide-derived carbon dots for non-viral co-delivery of different gene combinations and neuronal differentiation of ectodermal mesenchymal stem cells. Nanoscale. doi: 10.1039/C7NR03327C
Zhu, L., Ding, Y., Zhao, Q. (2017). Response gene to complement-32 mediates TGF-β-induced epithelial-mesenchymal transition via Erk-/p38-MAPK signaling pathways in human pancreatic cancer cell line BxPC-3. International Journal of Clinical and Experimental Pathology, 10(6), 7135-7143. Retrieved from http://www.ijcep.com/files/ijcep0052456.pdf
Yuan, Z., Li, D., Feng, P., Xue, G., Ji, C., Li, G., & Hölscher, C. (2017). A novel GLP-1/GIP dual agonist is more effective than liraglutide in reducing inflammation and enhancing GDNF release in the MPTP mouse model of Parkinson's disease. European Journal of Pharmacology. Advance online publication. doi: 10.1016/j.ejphar.2017.06.029
Wang, T., Liu, Z., Zhang, Z., Tang, S., Yue, M., Feng, S.,…, & Chen, Y. (2017). Evaluation of antitumor activity of survivin short interfering RNA delivered by lipid nanoparticles in colon cancer in vitro and in vivo. Oncology Letters, 14(2), 2001-2008. Advance online publication. doi: 10.3892/ol.2017.6404
Xu, J., Hu, C., Chen, S., Shen, H., Jiang, Q., Huang, P., & Zhao, W. (2017). Neuregulin-1 protects mouse cerebellum against oxidative stress and neuroinflammation. Brain Research, 1670, 32-43. Advance online publication. doi: 10.1016/j.brainres.2017.06.012
Zhang, Z., Wang, W., Jin, L., Cao, X., Jian, G., Wu, N.,…, & Wang, D. (2017). iTRAQ-based quantitative proteomics analysis of the protective effect of Yinchenwuling powder on hyperlipidemic rats. Evidence-Based Complementary and Alternative Medicine. Advance online publication. Retrieved from https://www.hindawi.com/journals/ecam/aip/3275096/
Zhang, J.H., Jiao, L.Y., Li, T.J., Zhu, Y.Y., Zhou, J.W., & Tian, J. (2017). GSK-3β suppresses HCC cell dissociation in vitro by upregulating epithelial junction proteins and inhibiting Wnt/β-catenin signaling pathway. Journal of Cancer, 8(9), 1598-1608. doi: 10.7150/jca.18744
Bai, L., Chu, G., Wang, W., Xiang, A., & Yang, G. (2017). BAMBI promotes porcine granulosa cell steroidogenesis involving TGF-β signaling. Theriogenology, 100, 24-31. doi: 10.1016/j.theriogenology.2017.05.022
Li, Z., Qian, R., Zhang, J., & Shi, X. (2017). Lipoma HMGIC fusion partner-like 3 (LHFPL3) promotes proliferation, migration and epithelial-mesenchymal transitions in human glioma cells. International Journal of Clinical and Experimental Pathology, 10(5), 5471-5479. Retrieved from http://www.ijcep.com/files/ijcep0048030.pdf
Chen, J., Wang, L., Lu, L., Shen, X., Huang, X.A., Liu, Y.,…, & Lei, H. (2017). Four Specific Hapten Conformations Dominating Antibody Specificity: Quantitative Structure–Activity Relationship Analysis for Quinolone Immunoassay. Analytical Chemistry. Advance online publication. doi: 10.1021/acs.analchem.7b00997
Chen, J., Liu, H., Gao, P., Hui, Y., Yang, Z., Zhang, X., …Fan, T. (2017). Preliminary evaluation for Bit1 as a potential biomarker for squamous cell carcinoma and adenocarcinoma of esophagus. Tumor Biology, 39(5). doi: 10.1177/1010428317708267
Liu, A., Yu, Q., Peng, Z., Huang, Y., Diao, S., Cheng, J.,…Hong, M. (2017). miR-200b inhibits CD133+ glioma cells by targeting the AKT pathway. Oncology Letters, 13(6), 4701-4707. doi: 10.3892/ol.2017.6055
Wang J, Tian W, Wang S, Wei W, Wu D, Wang H, Wang L, Yang R, Ji A, Li Y. Clin Exp Pharmacol Physiol. 2017 Apr 21. doi: 10.1111/1440-1681.12769. Anti-inflammatory and retinal protective effects of capsaicin on ischemia-induced injuries through the release of endogenous somatostatin.
Zhang, R., Yu, Q., Shi, G., Liu, R., Zhang, W., Zhao, X.,…, & Ge, M. (2017). chTLR4 pathway activation by Astragalus polysaccharide in bursa of Fabricius. BMC Veterinary Research, 13(1), 119. doi: 10.1186/s12917-017-1039-y.
Zhang L, Zhang H, Zhang H, Benson M, Han X, Li D. Food Chem Toxicol. 2017 Apr 13;105:177-185. doi: 10.1016/j.fct.2017.04.014. Roles of piRNAs in microcystin-leucine-arginine (MC-LR) induced reproductive toxicity in testis on male offspring.
Wei SY, Wang YX, Zhang QF, Zhao SL, Diao TT, Li JS, Qi WR, He YX, Guo XY, Zhang MZ, Chen JY, Wang XT, Wei QJ, Wang Y, Li B. Sci Rep. 2017 Apr 6;7:45952. doi: 10.1038/srep45952. Multiple Mechanisms are Involved in Salt-Sensitive Hypertension-Induced Renal Injury and Interstitial Fibrosis.
Song X, Hu D, Yan M, Wang Y, Wang N, Gu X, Yang G. Sci Rep. 2017 Mar 31;7(1):514. doi: 10.1038/s41598-017-00643-5. Molecular Characteristics and Serodiagnostic Potential of Dihydrofolate Reductase from Echinococcus granulosus.
Zheng S, Han F, Shi Y, Wen L, Han D. J Mol Neurosci. 2017 Mar 25. doi: 10.1007/s12031-017-0909-x.Single-Prolonged-Stress-Induced Changes in Autophagy-Related Proteins Beclin-1, LC3, and p62 in the Medial Prefrontal Cortex of Rats with Post-traumatic Stress Disorder.
H Guo, A Cao, S Chu, Y Wang, Y Zang, X Mao, H Wang… - Frontiers in Pharmacology, 2016 Astragaloside IV Attenuates Podocyte Apoptosis Mediated by Endoplasmic Reticulum Stress through Upregulating Sarco/Endoplasmic Reticulum Ca2+-ATPase 2 …
Nan Chen, Jingying Sun, Yali Song, Jian Ge, Yan Shi, Li Zhang Int J Clin Exp Pathol 2016;9(12):12219-12227 www.ijcep.com /ISSN:1936-2625/IJCEP0039429 Tripterygium wilfordii polyglycoside reduces the proliferation and inflammatory cytokines secretion of Hacat cells by regulating the balance of neutrophil elastase and trappin-2
Nan Chen1*, Jingying Sun2*, Yali Song1, Jian Ge3, Yan Shi1, Li Zhang1 Int J Clin Exp Pathol 2016;9(12):12219-12227 www.ijcep.com /ISSN:1936-2625/IJCEP0039429 Tripterygium wilfordii polyglycoside reduces the proliferation and inflammatory cytokines secretion of Hacat cells by regulating the balance of neutrophil elastase and trappin-2
Ying Wanga, Yang Yaob, Yuan Lic, Han Nied, Xingu Hea Neuroscience Letters Available online 15 December 2016 Prenatal morphine exposure during late embryonic stage enhances the rewarding effects of morphine and induces the loss of membrane-bound protein kinase C-α in intermediate medial mesopallium in the chick
Shuang-Shuang Zhanga, Zhou Wuc, Zhen Zhang, Zhou-Yi Xiongd, Hong Chenb, Qiao-Bing Huang Biochemical and Biophysical Research Communications Glucagon-like peptide-1 inhibits the receptor for advanced glycation endproducts to prevent podocyte apoptosis induced by advanced oxidative protein products
Yuling Li, Hong Li, Jing Zhang, Weikang Zhao, Jieliang Shena and Dianming Jiang RSC Adv., 2016,6, 114086-114095 In vitro evaluation of an yttria-stabilized zirconia reinforced nano-hydroxyapatite/polyamide 66 ternary biomaterial: biomechanics, biocompatibility and bioactivity
Li Z, Meng X, Jin L. Am J Transl Res. 2016 Nov 15;8(11):4628-4643. eCollection 2016. Icaritin induces apoptotic and autophagic cell death in human glioblastoma cells.

FAQs

Q: Is this product suitable for IHC? Keyword: application, Immunohistochemistry
A: Please beware that this product is not suitable for IHC. Small package of secondaries (0.5 ml) can do 15-75 slides and 1ml can do 30-150 slides. The difference is in the way customer uses the reagent.