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SKU BA1054
Pack size 0.5 ml
Applications Western blot
Price: $95.00

Product Overview

Product Name Goat Anti-Rabbit IgG Secondary Antibody, HRP Conjugate
Synonyms HRP-conjugated goat anti-rabbit IgG
Description Goat Anti-Rabbit IgG Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through Dot Blot, WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex.
Reagent Type Secondary antibody, reporter enzyme labeled
Label HRP (Horseradish Peroxidase)
Host Goat
Target Species Rabbit
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule rabbit IgG
Purification Immunoaffinity Chromatography
Specificity Rabbit IgG specific; No cross-reactivity with related species
Form Supplied Liquid: concentrated buffered stock solution
Formulation 0.5 mg HRP-conjugated secondary antibody
0.01 M PBS (PH 7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application ELISA*, WB*, Dot blot
*Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage 4C for 1 year
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Protein detection/quantification
Technique Immunodetection of target antibody with HRP reporter enzyme
Equipment Needed WB/Dot blot/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
Compatibility with Reagents Incompatible with sodium azide and metals
incompatible with high phosphate concentrations

Main Advantages

Specific High signal-to-noise ratio
Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast Generates strong signals in a relatively short time span
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

Goat Anti-Rabbit IgG Secondary Antibody, HRP Conjugate Images

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Goat Anti-Rabbit IgG Secondary Antibody, HRP Conjugate
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Publications

H Guo, A Cao, S Chu, Y Wang, Y Zang, X Mao, H Wang… - Frontiers in Pharmacology, 2016 Astragaloside IV Attenuates Podocyte Apoptosis Mediated by Endoplasmic Reticulum Stress through Upregulating Sarco/Endoplasmic Reticulum Ca2+-ATPase 2 …
Nan Chen, Jingying Sun, Yali Song, Jian Ge, Yan Shi, Li Zhang Int J Clin Exp Pathol 2016;9(12):12219-12227 www.ijcep.com /ISSN:1936-2625/IJCEP0039429 Tripterygium wilfordii polyglycoside reduces the proliferation and inflammatory cytokines secretion of Hacat cells by regulating the balance of neutrophil elastase and trappin-2
Nan Chen1*, Jingying Sun2*, Yali Song1, Jian Ge3, Yan Shi1, Li Zhang1 Int J Clin Exp Pathol 2016;9(12):12219-12227 www.ijcep.com /ISSN:1936-2625/IJCEP0039429 Tripterygium wilfordii polyglycoside reduces the proliferation and inflammatory cytokines secretion of Hacat cells by regulating the balance of neutrophil elastase and trappin-2
Ying Wanga, Yang Yaob, Yuan Lic, Han Nied, Xingu Hea Neuroscience Letters Available online 15 December 2016 Prenatal morphine exposure during late embryonic stage enhances the rewarding effects of morphine and induces the loss of membrane-bound protein kinase C-α in intermediate medial mesopallium in the chick
Shuang-Shuang Zhanga, Zhou Wuc, Zhen Zhang, Zhou-Yi Xiongd, Hong Chenb, Qiao-Bing Huang Biochemical and Biophysical Research Communications Glucagon-like peptide-1 inhibits the receptor for advanced glycation endproducts to prevent podocyte apoptosis induced by advanced oxidative protein products
Yuling Li, Hong Li, Jing Zhang, Weikang Zhao, Jieliang Shena and Dianming Jiang RSC Adv., 2016,6, 114086-114095 In vitro evaluation of an yttria-stabilized zirconia reinforced nano-hydroxyapatite/polyamide 66 ternary biomaterial: biomechanics, biocompatibility and bioactivity
Li Z, Meng X, Jin L. Am J Transl Res. 2016 Nov 15;8(11):4628-4643. eCollection 2016. Icaritin induces apoptotic and autophagic cell death in human glioblastoma cells.

FAQs

Q: Is this product suitable for IHC? Keyword: application, Immunohistochemistry
A: Please beware that this product is not suitable for IHC. Small package of secondaries (0.5 ml) can do 15-75 slides and 1ml can do 30-150 slides. The difference is in the way customer uses the reagent.