HSP90B (Phospho-Ser254) Colorimetric Cell-Based ELISA Kit

The HSP90B (Phospho-Ser254) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP90B (Phospho-Ser254) protein expression profile in cells. The kit can be used for measuring the relative amounts of HSP90B (Phospho-Ser254) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP90B (Phospho-Ser254).

Product Info Summary

SKU: EKC1943
Size: 1 kit, containing 2 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

HSP90B (Phospho-Ser254) Colorimetric Cell-Based ELISA Kit

See all HSP90 beta products

SKU/Catalog Number

EKC1943

Size

1 kit, containing 2 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The HSP90B (Phospho-Ser254) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP90B (Phospho-Ser254) protein expression profile in cells. The kit can be used for measuring the relative amounts of HSP90B (Phospho-Ser254) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP90B (Phospho-Ser254).

Storage & Handling

4°C/6 Months

Cite This Product

HSP90B (Phospho-Ser254) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1943)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1943 is reactive to HSP90AB1 in Human, Mouse, Rat samples

Application Guarantee

EKC1943 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-HSP90AB1 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-HSP90AB1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For HSP90AB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08238

Gene ID

3326

Gene Name

HSP90AB1

Full Name

Heat shock protein HSP 90-beta

Weight

83.264kDa

Superfamily

heat shock protein 90 family

Alternative Names

D6S182; Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein beta; heat shock protein HSP 90-beta; HSP 84; HSP84; HSP90 beta; HSP90AB1; HSP90B; HSP90-BETA; HSP90BHSP 90; HSPC2; HSPC2D6S182; HSPCB; HSPCBFLJ26984 HSP90AB1 D6S182, HSP84, HSP90B, HSPC2, HSPCB heat shock protein 90 alpha family class B member 1 heat shock protein HSP 90-beta|HSP90-beta|heat shock 84 kDa|heat shock 90kD protein 1, beta|heat shock protein 90 kDa|heat shock protein 90kDa alpha (cytosolic), class B member 1|heat shock protein 90kDa alpha family class B member 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HSP90AB1, check out the HSP90AB1 Infographic

HSP90AB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HSP90AB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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