Human 293T Whole Cell Lysate

Human 293T Whole Cell Lysate

Product Info Summary

Description: Human 293T Whole Cell Lysate
SKU: LYSATE0032
Applications: Western blot control (20-40μg/lane)
Size: 200μg

Overview

Product Name Human 293T Whole Cell Lysate
SKU: LYSATE0032
Description This whole cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Expression Profile: Read about Human 293T Whole Cell Lysate gene expression and protein transcription profile details:
Applications Western blot control (20-40μg/lane)
Quality Control 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
3. This lysate is used to test many Boster's primary antibodies. See QC images from these antibodies for more details

Properties

Form Liquid
Size 200μg
Concentration: 2μg/μl
Formulation: The cell lysate is in SDS-PAGE sample buffer, pH 6.8, containing 50mM TRIS-HCl, 2% SDS, 100mM DTT, 10% glycerol, and 0.01% bromophenol blue.
Storage Shipped at 4˚C. Aliquot and store at -20˚C or -80˚C upon delivery. Avoid repeated freezing and thawing.

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1 Customer Q&As for Human 293T Whole Cell Lysate

Question

Has lysate0032 been tested to express LRP2 by using M02463 antibody?

Verified customer

Asked: 2019-07-18

Answer

Yes, our lab technicians confirmed that the Human 293T Whole Cell Lysate LYSATE0032 expressed LRP2 by using Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody M02463 during our test.

Boster Scientific Support

Answered: 2019-07-18

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