Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

ADAMTS13 ELISA antibody pairs, Human, for 5 plates

Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Info Summary

SKU: EZ0927
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all ADAMTS13 products

SKU/Catalog Number

EZ0927

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0927)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q34-W688

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0927 is reactive to ADAMTS13 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EZ0927 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ0927-CA Rabbit anti- human ADAMTS13 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0927-DA Biotinylated goat anti- human ADAMTS13 polyclonal antibody (Detection Antibody) 500 µl, 10 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EK0927-ST Lyophilized recombinant human ADAMTS13 standard 50 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Incubator.
  • 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 5. Clean tubes and Eppendorf tubes.
  • 6. 96 well microplate (Cat# AR1100)
  • 7. Plate Sealers.
  • 8. Capture Antibody Diluent: PBS.
  • 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 12. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0790.2080.2950.4800.7331.2281.6492.001

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5.6210.125.56.2312.126.4
Standard deviation0.2590.4441.070.4670.8351.95
CV (%)4.6%4.4%4.2%7.5%6.9%7.4%

Gene/Protein Information For ADAMTS13 (Source: Uniprot.Org, NCBI)

Gene Name

ADAMTS13

Full Name

A disintegrin and metalloproteinase with thrombospondin motifs 13

Weight

153.604kDa

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; ADAMTS13; ADAM-TS13; ADAMTS-13; DKFZp434C2322; EC 3.4.24.14; EC 3.4.24.82; EC 3.4.24.87; FLJ42993; MGC118899; MGC118900; TTP; TTPADAM-TS 13; vWF-cleaving protease; vWF-CP; vWF-CPC9orf8; VWFCPvon Willebrand factor-cleaving protease ADAMTS13 ADAM-TS13, ADAMTS-13, C9orf8, VWFCP, vWF-CP ADAM metallopeptidase with thrombospondin type 1 motif 13 A disintegrin and metalloproteinase with thrombospondin motifs 13|a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13|vWF-cleaving protease|von Willebrand factor-cleaving protease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADAMTS13, check out the ADAMTS13 Infographic

ADAMTS13 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADAMTS13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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2 Customer Q&As for Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

Does EZ0927 kit able to recognise the murine ADAMTS13?

Verified customer

Asked: 2019-11-20

Answer

The Human ADAMTS13 ELISA Kit EZ-Set (DIY Antibody Pairs) EZ0927 ELISA could not recognise the murine ADAMTS13.

Boster Scientific Support

Answered: 2019-11-20

Question

Is the human plasma for the EZ0927 kit citrated or EDTA collection or Heparin collection?

Verified customer

Asked: 2019-07-20

Answer

The Human plasma for the Human ADAMTS13 ELISA Kit EZ-Set (DIY Antibody Pairs) EZ0927 was EDTA collection.

Boster Scientific Support

Answered: 2019-07-20

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