Product Info Summary
SKU: | EZ0927 |
---|---|
Size: | For 5 plates, 96 wells each |
Reactive Species: | Human |
Application: | ELISA |
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Product info
Product Name
Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ0927
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0927)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: Q34-W688
Assay Range
0.78 ng/ml - 50 ng/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0927 is reactive to ADAMTS13 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EZ0927 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0927-CA | Rabbit anti- human ADAMTS13 polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0927-DA | Biotinylated goat anti- human ADAMTS13 polyclonal antibody (Detection Antibody) | 500 µl, 10 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0927-ST | Lyophilized recombinant human ADAMTS13 standard | 50 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.079 | 0.208 | 0.295 | 0.480 | 0.733 | 1.228 | 1.649 | 2.001 |
Data Example Images
Click image to see more details
Human ADAMTS13 EZ Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 5.62 | 10.1 | 25.5 | 6.23 | 12.1 | 26.4 |
Standard deviation | 0.259 | 0.444 | 1.07 | 0.467 | 0.835 | 1.95 |
CV (%) | 4.6% | 4.4% | 4.2% | 7.5% | 6.9% | 7.4% |
Protein Target Info & Infographic
Gene/Protein Information For ADAMTS13 (Source: Uniprot.Org, NCBI)
Gene Name
ADAMTS13
Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 13
Weight
153.604kDa
Alternative Names
A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; ADAMTS13; ADAM-TS13; ADAMTS-13; DKFZp434C2322; EC 3.4.24.14; EC 3.4.24.82; EC 3.4.24.87; FLJ42993; MGC118899; MGC118900; TTP; TTPADAM-TS 13; vWF-cleaving protease; vWF-CP; vWF-CPC9orf8; VWFCPvon Willebrand factor-cleaving protease ADAMTS13 ADAM-TS13, ADAMTS-13, C9orf8, VWFCP, vWF-CP ADAM metallopeptidase with thrombospondin type 1 motif 13 A disintegrin and metalloproteinase with thrombospondin motifs 13|a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13|vWF-cleaving protease|von Willebrand factor-cleaving protease
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ADAMTS13, check out the ADAMTS13 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ADAMTS13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0927)
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Customer Q&As
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2 Customer Q&As for Human ADAMTS13 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
Question
Does EZ0927 kit able to recognise the murine ADAMTS13?
Verified customer
Asked: 2019-11-20
Answer
The Human ADAMTS13 ELISA Kit EZ-Set (DIY Antibody Pairs) EZ0927 ELISA could not recognise the murine ADAMTS13.
Boster Scientific Support
Answered: 2019-11-20
Question
Is the human plasma for the EZ0927 kit citrated or EDTA collection or Heparin collection?
Verified customer
Asked: 2019-07-20
Answer
The Human plasma for the Human ADAMTS13 ELISA Kit EZ-Set (DIY Antibody Pairs) EZ0927 was EDTA collection.
Boster Scientific Support
Answered: 2019-07-20