Human Adiponectin PicoKine™ Fast ELISA Kit


SKU FEK0595
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, serum, plasma(heparin, EDTA)and urine.
Sample Volume 100ul per well
Sensitivity <60pg/ml
Assay Range 1.56ng/ml-100ng/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Human Adiponectin PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK0595
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Adiponectin/ADIPOQ with <60pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA)and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Adiponectin/ADIPOQ tissue specificity:
Cite This Product Human Adiponectin PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0595)
Sample Type cell culture supernates, serum, plasma(heparin, EDTA)and urine. Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <60pg/ml
Assay Range 1.56ng/ml-100ng/ml
Immunogen Expression system for standard: NSO; Immunogen sequence: E19-N244
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
FEK0595-CAP 96-well plate precoated with anti-Human ADIPOQ antibody 1
FEK0595-ST lyophilized recombinant Human ADIPOQ standard 100ng/tube
FEK0595-DA biotinylated anti-Human ADIPOQ antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human Adiponectin PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(ng/ml)01.563.126.2512.52550100
O.D.0.0010.1390.2830.4950.8591.3081.8732.331

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3726120584992736461147849590
Standard deviation182.57940.522346.56236.991033.022578.68
CV(%)4.9%7.8%4.7%6.5%9%5.2%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13726381038493865381253.791.4%
Sample 21205812135116221035711543712.136.1%
Sample 349927481965275450855504331645.093.2%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id A8K660
Gene Name ADIPOQ
Protein Name adiponectin, C1Q and collagen domain containing
Alternative Names Adiponectin ;Adiponectin, C1Q and collagen domain containing ;cDNA FLJ78108, highly similar to Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA ;ADIPOQ ;hCG_1784052 ;
Molecular Weight 26414 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Human

*You can search these to find other products in these research areas.
Background Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226(19-244) amino acids with the molecular mass of 36KDa after glycosylation.

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FEK0595
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$480.00

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Altay C, Se?il M, Demir T, Atik T, Ak?nc? G, ?zdemir Kutbay N, Keskin Temelo?lu E, Y?ld?r?m ?im?ir I, ?z???k S, Demir L, Eren E, Tuna EB, Ayta? H, Onay H, Ak?nc? B. Diagn Interv Radiol. 2017 Nov-Dec;23(6):428-434. doi: 10.5152/dir.2017.17019. Dete...
Hatami Zargaran Z, Salehi M, Heydari St, Babajafari S. Int Cardiovasc Res J. 2014 Apr;8(2):52-6. Epub 2014 Apr 1. The Effects Of 6 Isocaloric Meals On Body Weight, Lipid Profiles, Leptin, And Adiponectin In Overweight Subjects (Bmi ≫ 25).
Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...
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