Human Aggrecan PicoKine™ Fast ELISA Kit


SKU FEK0909
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, serum and urine.
Sample Volume 100ul per well
Sensitivity <50pg/ml
Assay Range 1.56ng/ml-100ng/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Human Aggrecan PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK0909
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Aggrecan/ACAN with <50pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Aggrecan/ACAN tissue specificity: Restricted to cartilages. .
Cite This Product Human Aggrecan PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0909)
Sample Type cell culture supernates, serum and urine.
Sensitivity <50pg/ml
Assay Range 1.56ng/ml-100ng/ml
Immunogen NSO, V20-G675
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|monoclonal antibody from mouse

Assay Details

Kit Components

Catalog number Description Quantity
FEK0909-CAP 96-well plate precoated with anti-Human ACAN antibody 1
FEK0909-ST lyophilized recombinant Human ACAN standard 100ng/tube
FEK0909-DA biotinylated anti-Human ACAN antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human Aggrecan PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(ng/ml)01.563.126.2512.52550100
O.D.0.0010.0520.1090.2350.5121.0331.7872.427

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3084130644310233221398240208
Standard deviation240.55679.321939.59275.72908.831809.36
CV(%)7.8%5.2%4.5%8.3%6.5%4.5%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13084301131912954306088.562.8%
Sample 21306411683113381303612280779.416.3%
Sample 34310243347442894284743396544.91.2%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P16112
Gene Name ACAN
Protein Name Aggrecan core protein
Tissue Specificity Restricted to cartilages. .
Alternative Names Aggrecan core protein;Cartilage-specific proteoglycan core protein;CSPCP;Chondroitin sulfate proteoglycan core protein 1;Chondroitin sulfate proteoglycan 1;Aggrecan core protein 2;ACAN;AGC1, CSPG1, MSK16;
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Molecular Weight 250193 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Research Areas Human

*You can search these to find other products in these research areas.
Background Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.

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FEK0909
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$419.00

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