Product Info Summary
SKU: | EZ0305 |
---|---|
Size: | For 5 plates, 96 wells each |
Reactive Species: | Human |
Application: | ELISA |
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Product info
Product Name
Human Angiogenin/ANG ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ0305
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human Angiogenin/ANG ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ANG in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Angiogenin/ANG ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0305)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: Q25-P147
Assay Range
78 pg/ml - 5,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0305 is reactive to ANG in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Application Guarantee
EZ0305 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0305-CA | Rabbit anti- human ANG polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0305-DA | Biotinylated goat anti- human ANG polyclonal antibody (Detection Antibody) | 500 µl, 8.3 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0305-ST | Lyophilized recombinant human ANG standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.047 | 0.116 | 0.169 | 0.265 | 0.506 | 0.927 | 1.534 | 2.375 |
Data Example Images
Click image to see more details
Human Angiogenin/ANG EZ Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 312 | 672 | 1663 | 635 | 1856 | 3489 |
Standard deviation | 9.4 | 23.52 | 54.9 | 45.72 | 165.2 | 300 |
CV (%) | 3% | 3.5% | 3.3% | 7.2% | 8.9% | 8.6% |
Protein Target Info & Infographic
Gene/Protein Information For ANG (Source: Uniprot.Org, NCBI)
Gene Name
ANG
Full Name
Angiogenin
Weight
16.55kDa
Superfamily
pancreatic ribonuclease family
Alternative Names
ALS9; ANG; Angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4 ANG ALS9, HEL168, RAA1, RNASE4, RNASE5 angiogenin angiogenin|RNase 5|angiogenin, ribonuclease, RNase A family, 5|epididymis luminal protein 168|ribonuclease 5|ribonuclease A A1|ribonuclease A family member 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANG, check out the ANG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Angiogenin/ANG ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0305)
Hello CJ!
EZ0305 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing
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