Human Angiogenin/ANG ELISA Kit PicoKine®

Angiogenin ELISA kit for Human

Human Angiogenin/ANG ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. Cited in 6 publication(s).

Product Info Summary

SKU: EK0305
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Angiogenin/ANG ELISA Kit PicoKine®

View all Angiogenin ELISA kits

SKU/Catalog Number

EK0305

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Angiogenin/ANG ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Angiogenin/ANG ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0305)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q25-P147

Sensitivity

<12 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0305 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0305 is reactive to ANG in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0305 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Angiogenin

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Kit Components

Catalog Number Description Quantity
EK0305-CAP Anti-Human ANG Pre-coated 96-well strip microplate 1
EK0305-ST Human ANG Standard 2 vials, 10 ng/tube
EK0305-DA Human ANG Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156313625125025005000
O.D.0.0470.0900.1860.2800.4650.7061.1271.934

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Angiogenin, Dilution ratio of 1:200, concentration in serum and plasma is 156-524 ng/ml..

Some articles we found to cite concentrations of Angiogenin in samples: 24483841, 23553285 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Angiogenin/ANG ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10771929141007232957
Standard deviation4.8148.17195.234.649.16251.34
CV (%)4.5%6.7%6.7%4.6%6.8%8.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Angiogenin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11071211211091146.535.7%
Sample 271969763275570044.766.3%
Sample 32914284729622827288753.741.8%
*number of samples for each test n=16.

Gene/Protein Information For ANG (Source: Uniprot.Org, NCBI)

Gene Name

ANG

Full Name

Angiogenin

Weight

16.55kDa

Superfamily

pancreatic ribonuclease family

Alternative Names

ALS9; ANG; Angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4 ANG ALS9, HEL168, RAA1, RNASE4, RNASE5 angiogenin angiogenin|RNase 5|angiogenin, ribonuclease, RNase A family, 5|epididymis luminal protein 168|ribonuclease 5|ribonuclease A A1|ribonuclease A family member 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANG, check out the ANG Infographic

ANG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0305 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The association between serum angiogenin and osteopontin levels and coronary collateral circulation in patients with chronic total occlusion

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Li C, Fan J, Song X, Zhang B, Chen Y, Li C, Mi K, Ma H, Song Y, Tao X, Li G. Plos One. 2013 Sep 11;8(9):E75388. Doi: 10.1371/Journal.Pone.0075388. Ecollection 2013. Expression Of Angiopoietin-2 And Vascular Endothelial Growth Factor Receptor-3 Cor...

Shi H, Zhang Y, Fu S, Lu Z, Ye W, Xiao Y. Invest Ophthalmol Vis Sci. 2015 Jan 6;56(2):855-64. Doi: 10.1167/Iovs.14-15301. Angiotensin Ii As A Morphogenic Cytokine Stimulating Fibrogenesis Of Human Tenon'S Capsule Fibroblasts.

Bai X, Li X, Tian J, Zhou Z. Plos One. 2014 Apr 23;9(4):E96117. Doi: 10.1371/Journal.Pone.0096117. Ecollection 2014. Antiangiogenic Treatment Diminishes Renal Injury And Dysfunction Via Regulation Of Local Akt In Early Experimental Diabetes.

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8 Customer Q&As for Human Angiogenin/ANG ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-10-12

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-10-12

Question

Q: Can Angiogenin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

J. Chakrabarti

Verified customer

Asked: 2018-10-10

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-10-10

Question

Q: how to thaw whole blood sample for Angiogenin ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Angiogenin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-05-08

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-02-20

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-02-20

Question

Q: can I use citrate plasma as samples in Human Angiogenin Picokine® ELISA Kit (Catalog # EK0305)?

A. Anderson

Verified customer

Asked: 2018-02-10

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Angiogenin causing disruption of its protein structure. Angiogenin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Angiogenin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-02-10

Question

Q: if the enzyme conjugated Angiogenin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Angiogenin antigen?

Verified Customer

Verified customer

Asked: 2016-02-13

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-02-13

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Angiogenin using the EK0305 Human Angiogenin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2015-03-06

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2015-03-06

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Angiogenin in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2015-01-31

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Angiogenin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2015-01-31

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