Human Angiopoietin-1 / ANG1 PicoKine® Quick ELISA Kit

Angiopoietin-1 ELISA kit, 90-minute one-step protocol

Human Angiopoietin-1 / ANG1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPT1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: FEK0559
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 90 Minutes

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Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Angiopoietin-1 / ANG1 PicoKine® Quick ELISA Kit

See all Angiopoietin-1 products

SKU/Catalog Number

FEK0559

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Angiopoietin-1 / ANG1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPT1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Angiopoietin-1 / ANG1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0559)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q25-P147

Sensitivity

<12 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0559 is reactive to ANGPT1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0559 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Angiopoietin-1

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Kit Components

Catalog number Description Quantity
FEK0559-CAP 96-well plate precoated with anti-Human Angiopoietin-1 antibody 1
FEK0559-ST lyophilized recombinant Human Angiopoietin-1 standard 10 ng/tube
FEK0559-DA Biotinylated anti-Human Angiopoietin-1 detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0920.1180.2330.2330.3750.6841.2272.137

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2521375520127214405518
Standard deviation15.3790.75358.8617.68102.24452.47
CV (%)6.1%6.6%6.9%6.5%7.1%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11071211211091146.535.7%
Sample 271969763275570044.766.3%
Sample 32914284729622827288753.741.8%
*number of samples for each test n=16.

Gene/Protein Information For ANGPT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15389

Gene ID

284

Gene Name

ANGPT1

Full Name

Angiopoietin-1

Weight

57.513kDa

Alternative Names

AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003 ANGPT1 AGP1, AGPT, ANG1 angiopoietin 1 angiopoietin-1|ANG-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANGPT1, check out the ANGPT1 Infographic

ANGPT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANGPT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

FEK0559 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

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2 Customer Q&As for Human Angiopoietin-1 / ANG1 PicoKine® Quick ELISA Kit

Question

What is the difference between the Fast ELISA FEK0559 and the standard ELISA EK0559 kits? Is the assay procedure different?

Verified customer

Asked: 2019-09-30

Answer

The Human Angiogenin/ANG PicoKine™ Fast ELISA Kit (FEK0559) takes less assay time with two missing steps compared to the standard ELISA EK0559.

Boster Scientific Support

Answered: 2019-10-01

Question

What are the trade offs for faster times?

Verified customer

Asked: 2019-09-30

Answer

Fast ELISA kits requires more sensitive raw material and result in higher cost.

Boster Scientific Support

Answered: 2019-10-01

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