|Sample Type:||serum, plasma and cell culture supernates.|
Data & Images
|Product Name||Human Angiostatin K1-3 PicoKine™ ELISA Kit|
|Description||For quantitative detection of human Angiostatin K1-3 in serum, plasma and cell culture supernates.|
|Cite This Product||Human Angiostatin K1-3 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0905)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: NSO,V98-S355(K1-3)|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||serum, plasma and cell culture supernates.
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|EK0905-CAP||96-well plate precoated with anti-Human PLG antibody||1|
|EK0905-ST||lyophilized recombinant Human PLG standard||100ng/tubex2|
|EK0905-DA||biotinylated anti-Human PLG antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||90569 MW|
|Protein Function||Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. .|
|Tissue Specificity||Present in plasma and many other extracellular fluids. It is synthesized in the liver.|
|Sequence Similarities||Belongs to the peptidase S1 family. Plasminogen subfamily.|
|Subcellular Localization||Secreted . Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.|
|Alternative Names||Plasminogen;18.104.22.168;Plasmin heavy chain A;Activation peptide;Angiostatin;Plasmin heavy chain A, short form;Plasmin light chain B;PLG;|
|Research Areas|||cardiovascular|blood|fibrinolysis / thrombolysis| cardiovascular|platelets|coagulation|regulatory| cell biology|proteolysis / ubiquitin|proteolytic enzymes|other proteases|protease inhibitors|other protease inhibitors| kits/ lysates/ other|elisa kits|cancer proteins elisa kits|protease inhibitors elisa kits||
Background for Plasminogen
Human Angiostatin K1-3 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human Angiostatin K1-3 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 25-30min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.