Human APOA1/Apolipoprotein A-I ELISA Kit PicoKine®

Apolipoprotein A-I/ApoA1 ELISA kit for Human

Human APOA1 / Apoa I / Apolipoprotein AI ELISA Kit PicoKine™ (96 Tests). Quantitate Human APOA1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK1456
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human APOA1/Apolipoprotein A-I ELISA Kit PicoKine®

View all Apolipoprotein A-I/ApoA1 ELISA kits

SKU/Catalog Number

EK1456

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human APOA1 / Apoa I / Apolipoprotein AI ELISA Kit PicoKine™ (96 Tests). Quantitate Human APOA1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human APOA1/Apolipoprotein A-I ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1456)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D25-Q267

Sensitivity

<50 pg/ml

Assay Range

3.12 ng/ml - 200 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1456 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1456 is reactive to APOA1 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK1456 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Apolipoprotein A-I/ApoA1

Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Kit Components

Catalog Number Description Quantity
EK1456-CAP Anti-Human APOA1 Pre-coated 96-well strip microplate 1
EK1456-ST Human APOA1 Standard 2 vials, 200 ng/tube
EK1456-DA Human APOA1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)03.126.2512.52550100200
O.D.0.0670.1210.1700.2830.5221.0471.7381.899

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Apolipoprotein A-I/ApoA1, Dilution ratio of 1:200000, concentration in serum and plasma is around 5mg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human APOA1/Apolipoprotein A-I ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8186198269378081831918085400
Standard deviation376.551447.294876.56507.341514.625209.4
CV (%)4.6%7.3%5.2%6.2%7.9%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Apolipoprotein A-I/ApoA1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 181867996800273307878325.754.1%
Sample 21982619748206561965719971399.552%
Sample 393780862598732586965885823025.323.4%
*number of samples for each test n=16.

Gene/Protein Information For APOA1 (Source: Uniprot.Org, NCBI)

Gene Name

APOA1

Full Name

Apolipoprotein A-I

Weight

30.778kDa

Superfamily

apolipoprotein A1/A4/E family

Alternative Names

Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2 APOA1 HPALP2, apo(a) apolipoprotein A1 apolipoprotein A-I|apo-AI|epididymis secretory sperm binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on APOA1, check out the APOA1 Infographic

APOA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for APOA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1456 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma

Ding N, Luo S, Yu J, Zhou Y, Wu Z. Mol Vis. 2018 Mar 26;24:252-260. eCollection 2018. Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment

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10 Customer Q&As for Human APOA1/Apolipoprotein A-I ELISA Kit PicoKine®

Question

Q: What is the optimal O.D. value for Apolipoprotein A-I ELISA kit? I performed your Apolipoprotein A-I ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Apolipoprotein A-I even though the O.D. values are not very high?

B. Nelson

Verified customer

Asked: 2020-10-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Apolipoprotein A-I ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Apolipoprotein A-I that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2020-10-27

Question

EK1456 (Human apoa1) what are the suggested dilution ratios for the following sample types : cell culture supernates, serum, plasma(heparin, EDTA) and urine? The customer will probably need human plasma's dilution, but the customer didn't mention it for EK1456, so I would like to get the information for all the sample types just in case.

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

Please see the following suggested dilution ratios for the samples. EK1456 serum 1: 1000000 plasma(heparin, EDTA) 1: 1000000 urine 1:1 cell culture supernates depending on the cell type and cell status Please suggest the customer run serial dilution to figure out the optimal dilution ratio.

Boster Scientific Support

Answered: 2020-04-16

Question

Q: Can Apolipoprotein A-I ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

P. Edwards

Verified customer

Asked: 2020-01-27

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-01-27

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Apolipoprotein A-I using the EK1456 Human Apolipoprotein A-I Picokine® ELISA Kit?

E. Babu

Verified customer

Asked: 2019-07-04

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-07-04

Question

Regarding your ELISA kit EK1456, can you please advise whether or not this kit has been tested with other species

Verified Customer

Verified customer

Asked: 2019-04-11

Answer

Our lab technician does not sugget the customer detect mouse samples using EK1456.

Boster Scientific Support

Answered: 2019-04-11

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-04-10

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-04-10

Question

Q: how do I analyze ELISA data? I have performed Apolipoprotein A-I level in serum.

Verified Customer

Verified customer

Asked: 2017-10-10

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-10-10

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-06-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-06-19

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2016-01-06

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-01-06

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Apolipoprotein A-I in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2015-04-21

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Apolipoprotein A-I ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2015-04-21

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