Human B2M / beta 2 Microglobulin ELISA Kit PicoKine®

beta 2-Microglobulin ELISA kit for Human

Human B2M / beta 2 Microglobulin ELISA Kit PicoKine™ (96 Tests). Quantitate Human B2M in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml .

Product Info Summary

SKU: EK1691
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Product Name

Human B2M / beta 2 Microglobulin ELISA Kit PicoKine®

View all beta 2-Microglobulin ELISA kits

SKU/Catalog Number

EK1691

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human B2M / beta 2 Microglobulin ELISA Kit PicoKine™ (96 Tests). Quantitate Human B2M in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml .

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human B2M / beta 2 Microglobulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1691)

Clonality of Antibodies

See Datasheet for details

Immunogen

Target protein isolated from human plasma

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1691 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1691 is reactive to B2M in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Application Guarantee

EK1691 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of beta 2-Microglobulin

Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Kit Components

Catalog Number Description Quantity
EK1691-CAP Anti-Human B2M Pre-coated 96-well strip microplate 1
EK1691-ST Human B2M Standard 2 vials, 10 ng/tube
EK1691-DA Human B2M Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0490.1130.1840.2250.5230.7661.3282.053

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect beta 2-Microglobulin, Dilution ratio of 1:2000, concentration in serum and plasma is around 3.2 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human B2M / beta 2 Microglobulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11031070509731011034757
Standard deviation21.9762.06219.1726.9769.48285.42
CV (%)7.5%5.8%4.3%8.7%6.3%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of beta 2-Microglobulin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 129328430933730520.146.6%
Sample 21070110410061045105635.773.3%
Sample 350974645456945454714224.184.7%
*number of samples for each test n=16.

Gene/Protein Information For B2M (Source: Uniprot.Org, NCBI)

Gene Name

B2M

Full Name

Beta-2-microglobulin

Weight

13.715kDa

Superfamily

beta-2-microglobulin family

Alternative Names

B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin B2M IMD43 beta-2-microglobulin beta-2-microglobulin|beta chain of MHC class I molecules|beta-2-microglobin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on B2M, check out the B2M Infographic

B2M infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for B2M: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Human B2M / beta 2 Microglobulin ELISA Kit PicoKine®

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-10-23

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-10-23

Question

Q: can I use citrate plasma as samples in Human beta 2-Microglobulin Picokine® ELISA Kit (Catalog # EK1691)?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of beta 2-Microglobulin causing degradation of its protein structure. beta 2-Microglobulin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the beta 2-Microglobulin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-03-04

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement beta 2-Microglobulin using the EK1691 Human beta 2-Microglobulin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-01-19

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2020-01-19

Question

What is the protocol for urine sample and storage using EK1691 kit?

Verified customer

Asked: 2019-07-24

Answer

The Urine sample preparation protocol using Human B2M / beta 2 Microglobulin ELISA Kit PicoKine EK1691 are as follows: 1) Collect urine into sterile or disposable container (Fresh urine sample must be used immediately or saved to avoid reproduction of bacteria which produce endogenous HRP for giving potential false positive results). 2) Centrifuge sample at 10,000 x g for 1 min. 3) Assay immediately or aliquot supernatant and hold at -80°C. (Avoid freeze/thaw cycles).

Boster Scientific Support

Answered: 2019-07-24

Question

Q: how do I analyze ELISA data? I measured beta 2-Microglobulin level in plasma .

J. Perez

Verified customer

Asked: 2019-04-18

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-04-18

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

W. Anderson

Verified customer

Asked: 2019-04-03

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-04-03

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of beta 2-Microglobulin in Human plasma ? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-06-16

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the beta 2-Microglobulin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-06-16

Question

Q: Can beta 2-Microglobulin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-04-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-04-11

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