Human B7-1/CD80 PicoKine™ Fast ELISA Kit


SKU FEK0707
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates and serum.
Sample Volume 100ul per well
Sensitivity <10pg/ml
Assay Range 62.5pg/ml-4000pg/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Human B7-1/CD80 PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK0707
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human B7-1/CD80 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. B7-1/CD80 tissue specificity: Expressed on activated B-cells, macrophages and dendritic cells.
Cite This Product Human B7-1/CD80 PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0707)
Sample Type cell culture supernates and serum.
Sensitivity <10pg/ml
Assay Range 62.5pg/ml-4000pg/ml
Immunogen Expression system for standard: NSO; Immunogen sequence: V35-N242
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|monoclonal antibody from mouse

Assay Details

Kit Components

Catalog number Description Quantity
FEK0707-CAP 96-well plate precoated with anti-Human CD80 antibody 1
FEK0707-ST lyophilized recombinant Human CD80 standard 10ng/tube
FEK0707-DA biotinylated anti-Human CD80 antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human B7-1/CD80 PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(pg/ml)04000200040002000400020004000
O.D.0.0060.0600.1290.2560.4420.8291.3841.979

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)16244516561634251791
Standard deviation7.2934.7169.557.9834100.29
CV(%)4.5%7.8%4.2%4.9%8%5.6%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 116213816413915012.278.1%
Sample 244549551545747828.235.9%
Sample 316561667140215601571106.216.7%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P33681
Gene Name CD80
Protein Name T-lymphocyte activation antigen CD80
Tissue Specificity Expressed on activated B-cells, macrophages and dendritic cells.
Alternative Names T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 33048 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .
Research Areas Human

*You can search these to find other products in these research areas.
Background The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

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FEK0707
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$419.00

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