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SKU:EZ0884
Pack Size:For 5 plates, 96 wells each
Validated Species:Human
Application:ELISA
Sensitivity:<10pg/ml
Assay Range:31.2pg/ml-2000pg/ml
Sample Type:bone tissue, cell culture supernates, serum, plasma((heparin, EDTA) and urine.
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Product Features:

Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

Overview

Product Name Human BMP-7 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
SKU/Catalog Number EZ0884
Description A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BMP-7 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Cite This Product Human BMP-7 EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0884)
Validated Species Human
Application ELISA

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Immunogen Expression system for standard: NSO; Immunogen sequence: S293-H431
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Pack Size For 5 plates, 96 wells each

Properties

Sensitivity <10pg/ml
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range 31.2pg/ml-2000pg/ml
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type bone tissue, cell culture supernates, serum, plasma((heparin, EDTA) and urine.

*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Capture Antibody monoclonal antibody from mouse
Detection Antibody polyclonal antibody from goat
Normal Levels Some research articles suggesting the natural levels of BMP7 are listed below:
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Kit Components

Catalog numberDescriptionQuantity
EZ0884-CACapture Antibody For BMP-7500ul
EZ0884-DABiotinylated Detection Antibody For BMP-7500ul
ST0000-10lyophilized recombinant human Lipocalin-2/BMP-7 standard10ng/tubex2
AR1103Avidin-Biotin-Peroxidase Complex(ABC)500ul(dilution 1:100)

Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.

*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.

See Prices For Extra Components

Material Required But Not Provided

Components Included In The EZ Set Accessory Kit

  • Antibody diluent buffer
  • ABC diluent buffer
  • Sample diluent buffer
  • TMB
  • TMB stop solution
  • 5x 96 well plates, plate sealers

*The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you.

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

    Material Required But Not Provided

    Components Included In The EZ Set Accessory Kit (EZA001)

    • Antibody diluent buffer
    • ABC diluent buffer
    • Sample diluent buffer
    • TMB
    • TMB stop solution
    • 5x 96 well plates, plate sealers

    *The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other sources if it is of convenience to you.

    Components And Equipment Not Included In The EZ Set Accessory Kit

    • 1. Microplate reader in standard size.
    • 2. Automated plate washer.
    • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
    • 4. Clean tubes and Eppendorf tubes.
    *Reagents needed for sample collection and preparation are not included.

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name BMP7
Protein Name Bone morphogenetic protein 7
Molecular Weight 49313 MW
Uniprot ID A8K571
Alternative Names Bone morphogenetic protein 7 (Osteogenic protein 1), isoform CRA_b ;cDNA FLJ78019, highly similar to Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1), mRNA ;cDNA, FLJ92758, Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1)(BMP7), mRNA ;BMP7 ;hCG_40100 ;
Research Areas |signal transduction|growth factors/hormones|tgf| signal transduction|cytoskeleton / ecm|extracellular matrix|structures|bone| cell biology|cell cycle|cell differentiation| stem cells|signaling pathways|tgf beta|secreted| cancer| developmental biology|organogenesis|nervous system development|excretory system development|kidney development| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Bone morphogenetic protein 7

Bone morphogenetic protein 7 or BMP7(also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Human BMP-7 EZ-Set™ ELISA Kit (DIY Antibody Pairs) Images

Click the images to enlarge.

Human BMP-7 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
Human BMP-7 EZ Set ELISA Kit standard curve

Intra/Inter Assay Precision

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Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n161616242424
Mean(pg/ml)34276511083727801265
Standard deviation19.842.857.630.562.498.7
CV(%)5.85.65.28.287.8

Typical Data Obtained from Human BMP-7 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

(TMB reaction incubate at 37°C for 20-25min)

Concentration(pg/ml)031.262.512525050010002000
O.D.0.0190.0840.1430.2910.4850.9791.7462.576

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

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