Product Info Summary
SKU: | EK2145 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine |
Product info
Product Name
Human C3a anaphylatoxin ELISA Kit PicoKine®
SKU/Catalog Number
EK2145
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human C3a anaphylatoxin ELISA Kit PicoKine™ (96 Tests). Quantitate Human C3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human C3a anaphylatoxin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2145)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S672-R748
Sensitivity
<20 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2145 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2145 is reactive to C3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine
Application Guarantee
EK2145 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of C3
Complement component 3, often simply called C3, is a protein of the immune system. In humans it is encoded on chromosome 19 by a gene called C3. Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2145-CAP | Anti-Human C3 Pre-coated 96-well strip microplate | 1 |
EK2145-ST | Human C3 Standard | 2 vials, 10 ng/tube |
EK2145-DA | Human C3 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
O.D. | 0.005 | 0.041 | 0.084 | 0.178 | 0.355 | 0.704 | 1.109 | 2.147 |
Data Example Images
Click image to see more details
Human C3a anaphylatoxin PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect C3, Dilution ratio of 1:2000000, concentration in serum and plasma is 936-1300 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human C3a anaphylatoxin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 63 | 213 | 1095 | 60 | 224 | 1088 |
Standard deviation | 3.28 | 14.06 | 50.37 | 3.06 | 14.34 | 38.08 |
CV(%) | 5.2% | 6.6% | 4.6% | 5.1% | 6.4% | 3.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of C3 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 63 | 61 | 62 | 65 | 63 | 3.83 | 6.1% |
Sample 2 | 213 | 195 | 232 | 215 | 214 | 11.97 | 5.6% |
Sample 3 | 1095 | 1045 | 1002 | 989 | 1033 | 58.87 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For C3 (Source: Uniprot.Org, NCBI)
Gene Name
C3
Full Name
Complement C3
Weight
187.148kDa
Alternative Names
AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; complement C3; complement component 3; complement component C3; CPAMD1acylation-stimulating protein cleavage product C3 AHUS5, ARMD9, ASPa, C3b, CPAMD1, HEL-S-62p, C3 complement C3 complement C3|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1|C3a anaphylatoxin|acylation-stimulating protein cleavage product|complement component 3|complement component C3a|complement component C3b|epididymis secretory sperm binding protein Li 62p|prepro-C3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on C3, check out the C3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for C3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human C3a anaphylatoxin ELISA Kit PicoKine® (EK2145)
Hello CJ!
EK2145 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of Astragalus membranaceus roots supplementation on growth performance, serum antioxidant and immune response in finishing lambs
Osteoclast differentiation and formation induced by titanium implantation through complement C3a
Effect of preoperative inhaled budesonide on pulmonary injury after cardiopulmonary bypass: A randomized pilot study
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