Human C3a anaphylatoxin ELISA Kit PicoKine™

Human C3a anaphylatoxin ELISA Kit PicoKine™ (96 Tests). Quantitate Human C3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml.

Product Info Summary

SKU: EK2145
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine

Product Name

Human C3a anaphylatoxin ELISA Kit PicoKine™

See all C3 products

SKU/Catalog Number

EK2145

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human C3a anaphylatoxin ELISA Kit PicoKine™ (96 Tests). Quantitate Human C3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human C3a anaphylatoxin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2145)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S672-R748

Sensitivity

<20 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2145 is reactive to C3 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine

Application Guarantee

EK2145 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2145-CAP Anti-Human C3 Pre-coated 96-well strip microplate 1
EK2145-ST Human C3 Standard 2 vials, 10 ng/tube
EK2145-DA Human C3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)031.262.51252505001,0002,000
O.D.0.0050.0410.0840.1780.3550.7041.1092.147

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human C3a anaphylatoxin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)632131095602241088
Standard deviation3.2814.0650.373.0614.3438.08
CV(%)5.2%6.6%4.6%5.1%6.4%3.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of C3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 163616265633.836.1%
Sample 221319523221521411.975.6%
Sample 3109510451002989103358.875.7%
*number of samples for each test n=16.

Gene/Protein Information For C3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01024

Gene ID

718

Gene Name

C3

Full Name

Complement C3

Weight

187.148kDa

Alternative Names

AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; complement C3; complement component 3; complement component C3; CPAMD1acylation-stimulating protein cleavage product C3 AHUS5, ARMD9, ASPa, C3b, CPAMD1, HEL-S-62p, C3 complement C3 complement C3|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1|C3a anaphylatoxin|acylation-stimulating protein cleavage product|complement component 3|complement component C3a|complement component C3b|epididymis secretory sperm binding protein Li 62p|prepro-C3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on C3, check out the C3 Infographic

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