|Sample Type:||cell culture supernates, serum and plasma(heparin).|
Data & Images
|Product Name||Human CD163 PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD163|
|Cite This Product||Human CD163 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0715)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: G41-S1045|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, serum and plasma(heparin).
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Natural Levels||Some research articles suggesting the natural levels of CD163 are listed below:|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|-||96-well plate precoated with anti- human CD163 antibody||1|
|ST0000-100||lyophilized recombinant human CD163 standard||100ng/tubex2|
|AR1107||biotinylated anti- human CD163 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Scavenger receptor cysteine-rich type 1 protein M130|
|Gene Full Name||Scavenger receptor cysteine-rich type 1 protein M130|
|Protein Function||Acute phase-regulated receptor involved in clearance andendocytosis of hemoglobin/haptoglobin complexes by macrophages andmay thereby protect tissues from free hemoglobin-mediatedoxidative damage. May play a role in the uptake and recycling ofiron, via endocytosis of hemoglobin/haptoglobin and subsequentbreakdown of heme. Binds hemoglobin/haptoglobin complexes in acalcium-dependent and pH-dependent manner. Exhibits a higheraffinity for complexes of hemoglobin and multimeric haptoglobin ofHP*1F phenotype than for complexes of hemoglobin and dimerichaptoglobin of HP*1S phenotype. Induces a cascade of intracellularsignals that involves tyrosine kinase-dependent calciummobilization, inositol triphosphate production and secretion ofIL6 and CSF1. Isoform 3 exhibits the higher capacity for ligandendocytosis and the more pronounced surface expression whenexpressed in cells.|
|Tissue Specificity||Expressed in monocytes and mature macrophagessuch as Kupffer cells in the liver, red pulp macrophages in thespleen, cortical macrophages in the thymus, resident bone marrowmacrophages and meningeal macrophages of the central nervoussystem. Expressed also in blood. Isoform 1 is the lowest abundantin the blood. Isoform 2 is the lowest abundant in the liver andthe spleen. Isoform 3 is the predominant isoform detected in theblood..|
|Sequence Similarities||Contains 9 SRCR domains.|
|Subcellular Localization||Soluble CD163: Secreted.|
|Uniprot ID||CD163: Q86VB7
|Alternative Names||Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; CD163; Soluble CD163; sCD163; CD163; M130;|
|Research Areas||immunology|innate immunity|macrophage / inflamm.|cell type markers|cd|myeloid cells|cardiovascular|atherosclerosis|vascular inflammation|innate and adaptive immunity||
Background for Scavenger receptor cysteine-rich type 1 protein M130
Human CD163 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human CD163 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-20min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.