Human CD21/CR2 PicoKine™ Fast ELISA Kit


SKU FEK1315
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Volume 100ul per well
Sensitivity <20pg/ml
Assay Range 0.78ng/ml-50ng/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Human CD21/CR2 PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK1315
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Cd21/CR2 with <20pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cd21/CR2 tissue specificity: Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
Cite This Product Human CD21/CR2 PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1315)
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <20pg/ml
Assay Range 0.78ng/ml-50ng/ml
Immunogen Expression system for standard: CHO; Immunogen sequence: Ile21-71
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
FEK1315-CAP 96-well plate precoated with anti-Human CR2 antibody 1
FEK1315-ST lyophilized recombinant Human CR2 standard 50ng/tube
FEK1315-DA biotinylated anti-Human CR2 antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human CD21/CR2 PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(ng/ml)00.781.563.126.2512.52550
O.D.0.0030.3550.6651.0381.4941.9722.2102.198

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)12427916240121177791025042
Standard deviation70.79372.051296.6489.45411.321627.73
CV(%)5.7%4.7%5.4%7.6%5.2%6.5%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11242146812731362133687.896.5%
Sample 279167621654868327229558.347.7%
Sample 324012232472646722418240361512.516.2%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P20023
Gene Name CR2
Protein Name Complement receptor type 2
Tissue Specificity Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
Alternative Names Complement receptor type 2;Cr2;Complement C3d receptor;Epstein-Barr virus receptor;EBV receptor;CD21;CR2;C3DR;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 112916 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. .
Research Areas Human

*You can search these to find other products in these research areas.
Background Complement receptor type 2(CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b(inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).

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FEK1315
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