Human CD25/IL-2sR Alpha PicoKine™ Fast ELISA Kit


SKU FEK0400
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Volume 100ul per well
Sensitivity <5pg/ml
Assay Range 78pg/ml-5000pg/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Human CD25/IL-2sR Alpha PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK0400
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Il-2 R Alpha/IL2RA with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il-2 R Alpha/IL2RA tissue specificity:
Cite This Product Human CD25/IL-2sR Alpha PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0400)
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <5pg/ml
Assay Range 78pg/ml-5000pg/ml
Immunogen Expression system for standard: NSO,E22-C213
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
FEK0400-CAP 96-well plate precoated with anti-Human IL2RA antibody 1
FEK0400-ST lyophilized recombinant Human IL2RA standard 10ng/tube
FEK0400-DA biotinylated anti-Human IL2RA antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human CD25/IL-2sR Alpha PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(pg/ml)078156313625125025005000
O.D.0.0160.2510.4350.7701.1081.4791.8532.049

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)14071923531417852310
Standard deviation6.5841.794.127.6147.197.02
CV(%)4.7%5.8%4%5.4%6%4.2%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 114011613911512712.019.4%
Sample 271973561670969446.46.6%
Sample 32353248424992400243460.082.4%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P01589
Gene Name IL2RA
Protein Name Interleukin-2 receptor subunit alpha
Alternative Names Interleukin-2 receptor subunit alpha;IL-2 receptor subunit alpha;IL-2-RA;IL-2R subunit alpha;IL2-RA;TAC antigen;p55;CD25;IL2RA;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 30819 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Receptor for interleukin-2.
Research Areas Human

*You can search these to find other products in these research areas.
Background CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

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FEK0400
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$419.00

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