|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine.|
Data & Images
|Product Name||Human CD26/DPP4 PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD26/DPP4|
|Cite This Product||Human CD26/DPP4 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0696)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: D34-P766|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine.
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Natural Levels||Some research articles suggesting the natural levels of DPP4 are listed below:|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|-||96-well plate precoated with anti- human CD26/DPP4 antibody||1|
|ST0000-20||lyophilized recombinant human CD26/DPP4 standard||20ng/tubex2|
|AR1107||biotinylated anti- human CD26/DPP4 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Dipeptidyl peptidase 4|
|Gene Full Name||Dipeptidyl peptidase 4|
|Protein Function||Cell surface glycoprotein receptor involved in thecostimulatory signal essential for T-cell receptor (TCR)-mediatedT-cell activation. Acts as a positive regulator of T-cellcoactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Itsbinding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Itsinteraction with ADA also regulates lymphocyte-epithelial celladhesion. In association with FAP is involved in the pericellularproteolysis of the extracellular matrix (ECM), the migration andinvasion of endothelial cells into the ECM. May be involved in thepromotion of lymphatic endothelial cells adhesion, migration andtube formation. When overexpressed, enhanced cell proliferation, aprocess inhibited by GPC3. Acts also as a serine exopeptidase witha dipeptidyl peptidase activity that regulates variousphysiological processes by cleaving peptides in the circulation,including many chemokines, mitogenic growth factors, neuropeptidesand peptide hormones. Removes N-terminal dipeptides sequentiallyfrom polypeptides having unsubstituted N-termini provided that thepenultimate residue is proline..|
|Tissue Specificity||Expressed specifically in lymphatic vesselsbut not in blood vessels in the skin, small intestine, esophagus,ovary, breast and prostate glands. Not detected in lymphaticvessels in the lung, kidney, uterus, liver and stomach (at proteinlevel). Expressed in the poorly differentiated crypt cells of thesmall intestine as well as in the mature villous cells. Expressedat very low levels in the colon..|
|Sequence Similarities||Belongs to the peptidase S9B family. DPPIV subfamily.|
|Subcellular Localization||Dipeptidyl peptidase 4 soluble form:Secreted. Detected in the serum and the seminal fluid.|
|Uniprot ID||DPP4: P27487
|Alternative Names||Dipeptidyl peptidase 4; 184.108.40.206; ADABP; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; TP103; CD26; Dipeptidyl peptidase 4 membrane form; Dipeptidyl peptidase IV membrane form; Dipeptidyl peptidase 4 soluble form; Dipeptidyl peptidase IV soluble form; DPP4; ADCP2, CD26;|
|Research Areas||kits/ lysates/ other|kits|cell metabolism kits|sugar assays||
Background for Dipeptidyl peptidase 4
Human CD26/DPP4 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human CD26/DPP4 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.