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Product Info Summary
|Size:||For 5 plates, 96 wells each|
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Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FCGR2B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1312)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A46-P217
46.9 pg/ml - 3,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ1312 is reactive to FCGR2B in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EZ1312 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EZ1312-CA||Rabbit anti- human FCGR2B/C polyclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ1312-DA||Biotinylated goat anti- human FCGR2B/C polyclonal antibody (Detection Antibody)||500 µl, 6 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ1312-ST||Lyophilized recombinant human FCGR2B/C standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CD32/FCGR2b/c EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
Gene/Protein Information For FCGR2B (Source: Uniprot.Org, NCBI)
Low affinity immunoglobulin gamma Fc region receptor II-b
CD32 antigen; CD32b; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIB; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FCGR2B; FcgRIIB; FCRIIB; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b FCGR2B CD32, CD32B, FCG2, FCGR2, FCGR2C, FcRII-c, IGFR2 Fc fragment of IgG receptor IIb low affinity immunoglobulin gamma Fc region receptor II-b|CDw32|Fc fragment of IgG, low affinity II, receptor for (CD32)|Fc fragment of IgG, low affinity IIb, receptor (CD32)|Fc fragment of IgG, low affinity IIb, receptor for (CD32)|Fc gamma RIIb|Fc gamma receptor IIb|Fc-gamma RII-c|Fc-gamma-RIIc|IgG Fc receptor II-c|Low affinity immunoglobulin gamma Fc region receptor II-c|fc-gamma RII-b|fc-gamma-RIIb|fcRII-b|igG Fc receptor II-b*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FCGR2B, check out the FCGR2B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCGR2B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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