|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA) and urine.|
Data & Images
|Product Name||Human Chitinase 3-like 1/YKL-40 PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Chitinase 3-like 1/YKL-40. 96wells/kit, with removable strips.|
|Cite This Product||Human Chitinase 3-like 1/YKL-40 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0974)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: Y22-T383|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and urine.
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Natural Levels||Some research articles suggesting the natural levels of CHI3L1 are listed below:|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|EK0974-CAP||96-well plate precoated with anti-Human CHI3L1 antibody||1|
|EK0974-ST||lyophilized recombinant Human CHI3L1 standard||10ng/tubex2|
|EK0974-DA||biotinylated anti-Human CHI3L1 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
|AR0030-E||PBS washing buffer||Powder for 1000ml|
Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Chitinase-3-like protein 1|
|Molecular Weight||42625 MW|
|Protein Function||Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. .|
|Tissue Specificity||Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. .|
|Sequence Similarities||Belongs to the glycosyl hydrolase 18 family.|
|Subcellular Localization||Secreted, extracellular space . Cytoplasm . Cytoplasm, perinuclear region . Endoplasmic reticulum .|
|Alternative Names||Chitinase-3-like protein 1;39 kDa synovial protein;Cartilage glycoprotein 39;CGP-39;GP-39;hCGP-39;YKL-40;CHI3L1;|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|other ecm proteins||
Background for Chitinase-3-like protein 1
Human Chitinase 3-like 1/YKL-40 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human Chitinase 3-like 1/YKL-40 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-20min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.