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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human CLEC1B ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CLEC1B ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLEC1B in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CLEC1B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1921)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S55-P229
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1921 is reactive to CLEC1B in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1921 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CLEC-2/CLEC-1B
C-type lectin domain family 1 member B is a protein that in humans is encoded by the CLEC1B gene. This gene is mapped to 12p13.31-p13.2. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells.
|EK1921-CAP||Anti-Human CLEC1B Pre-coated 96-well strip microplate||1|
|EK1921-ST||Human CLEC1B Standard||2 vials, 10 ng/tube|
|EK1921-DA||Human CLEC1B Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CLEC1B PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CLEC-2/CLEC-1B, Dilution ratio of 1:1 1:5, concentration in serum is around 1000pg/ml around 4000pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CLEC1B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CLEC-2/CLEC-1B in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CLEC1B (Source: Uniprot.Org, NCBI)
C-type lectin domain family 1 member B
1810061I13Rik; CLEC1B; CLEC2; CLEC-2; CLEC2B; CLEC2PRO1384; C-type lectin domain family 1 member B; C-type lectin domain family 1, member B; C-type lectin-like receptor 2; C-type lectin-like receptor-2; QDED721 CLEC1B 1810061I13Rik, CLEC2, CLEC2B, PRO1384, QDED721 C-type lectin domain family 1 member B C-type lectin domain family 1 member B|C-type lectin-like receptor 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CLEC1B, check out the CLEC1B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CLEC1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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