Human Complement Factor H/CFH ELISA Kit PicoKine®

Complement Factor H ELISA kit for Human

Human Complement Factor H/CFH ELISA Kit PicoKine™ (96 Tests). Quantitate Human CFH in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1399
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human Complement Factor H/CFH ELISA Kit PicoKine®

View all Complement Factor H ELISA kits

SKU/Catalog Number

EK1399

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Complement Factor H/CFH ELISA Kit PicoKine™ (96 Tests). Quantitate Human CFH in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Complement Factor H/CFH ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1399)

Clonality of Antibodies

See Datasheet for details

Immunogen

Target protein isolated from human plasma

Sensitivity

<50 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1399 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1399 is reactive to CFH in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK1399 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Complement Factor H

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Kit Components

Catalog Number Description Quantity
EK1399-CAP Anti-Human CFH Pre-coated 96-well strip microplate 1
EK1399-ST Human CFH Standard 2 vials, 100 ng/tube
EK1399-DA Human CFH Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.0170.1350.2330.4450.8571.3431.8132.017

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Complement Factor H, Dilution ratio of 1:20000, concentration in serum and plasma is around 300 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Complement Factor H/CFH ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3203135185384434061293557149
Standard deviation233.81784.043822.92303.13905.454971.96
CV (%)7.3%5.8%7.1%8.9%7%8.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Complement Factor H in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13203299431703050310485.42.7%
Sample 21351812944134671171412910726.545.6%
Sample 35384453833530945268053362497.770.9%
*number of samples for each test n=16.

Gene/Protein Information For CFH (Source: Uniprot.Org, NCBI)

Gene Name

CFH

Full Name

Complement factor H

Weight

139.096kDa

Alternative Names

adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin ; beta-1-H-globulin; CFH; CFHL3; Complement Factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 1; H factor 2 (complement); HF; HF1; HF1ARMS1; HF2; HUS; HUSMGC88246 CFH AHUS1, AMBP1, ARMD4, ARMS1L3, FH, FHL1, HF, HF1, HF2, HUS, CFH complement factor H complement factor H|H factor 1 (complement)|H factor 2 (complement)|adrenomedullin binding protein|age-related maculopathy susceptibility 1|beta-1-H-globulin|beta-1H|factor H|factor H-like 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CFH, check out the CFH Infographic

CFH infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CFH: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1399 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Comprehensive Proteomic Profiling of Patients’ Tears Identifies Potential Biomarkers for the Traumatic Vegetative State

Have you used Human Complement Factor H/CFH ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human Complement Factor H/CFH ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Human Complement Factor H/CFH ELISA Kit PicoKine®

Question

Q: is it okay to use citrate plasma as samples in Human Complement Factor H Picokine® ELISA Kit (Catalog # EK1399)?

K. Zhang

Verified customer

Asked: 2020-10-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Complement Factor H causing disruption of its protein structure. Complement Factor H may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Complement Factor H ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-10-26

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-08-30

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-08-30

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

W. Miller

Verified customer

Asked: 2020-06-12

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-06-12

Question

Q: can you recommend the dilution ratio of serum samples for detection of Complement Factor H in Human urine? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

S. Chakrabarti

Verified customer

Asked: 2020-03-07

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Complement Factor H ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-03-07

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Complement Factor H level in urine.

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-09-06

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-16

Question

Q: if the enzyme conjugated Complement Factor H antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Complement Factor H antigen?

Y. Li

Verified customer

Asked: 2018-06-12

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-06-12

$499
In stock, 1 left.

Order within -5 hours and -35 minutes to receive by Fri Mar 29

Get A Quote
Eddy test
In stock
Order Product
EK1399
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.