|Sample Type:||cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).|
Data & Images
|Product Name||Human CSF1R/M-CSFR PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CSF1R/M-CSFR|
|Cite This Product||Human CSF1R/M-CSFR PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0807)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: I20-E512|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|-||96-well plate precoated with anti- human CSF1R/M-CSFR antibody||1|
|ST0000-10||lyophilized recombinant human CSF1R/M-CSFR standard||10ng/tubex2|
|AR1107||biotinylated anti- human CSF1R/M-CSFR antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Macrophage colony-stimulating factor 1 receptor|
|Gene Full Name||Macrophage colony-stimulating factor 1 receptor|
|Protein Function||Tyrosine-protein kinase that acts as cell-surfacereceptor for CSF1 and IL34 and plays an essential role in theregulation of survival, proliferation and differentiation ofhematopoietic precursor cells, especially mononuclear phagocytes,such as macrophages and monocytes. Promotes the release ofproinflammatory chemokines in response to IL34 and CSF1, andthereby plays an important role in innate immunity and ininflammatory processes. Plays an important role in the regulationof osteoclast proliferation and differentiation, the regulation ofbone resorption, and is required for normal bone and toothdevelopment. Required for normal male and female fertility, andfor normal development of milk ducts and acinar structures in themammary gland during pregnancy. Promotes reorganization of theactin cytoskeleton, regulates formation of membrane ruffles, celladhesion and cell migration, and promotes cancer cell invasion.Activates several signaling pathways in response to ligandbinding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL.Activation of PLCG2 leads to the production of the cellularsignaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinaseC family members, especially PRKCD. Phosphorylation of PIK3R1, theregulatory subunit of phosphatidylinositol 3-kinase, leads toactivation of the AKT1 signaling pathway. Activated CSF1R alsomediates activation of the MAP kinases MAPK1/ERK2 and/orMAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1.Activated CSF1R transmits signals both via proteins that directlyinteract with phosphorylated tyrosine residues in itsintracellular domain, or via adapter proteins, such as GRB2.Promotes activation of STAT family members STAT3, STAT5A and/orSTAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases,such as INPP5D/SHIP-1, that dephosphorylate the receptor and itsdownstream effectors, and by rapid internalization of theactivated receptor..|
|Tissue Specificity||Expressed in bone marrow and in differentiatedblood mononuclear cells.|
|Sequence Similarities||Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.|
|Subcellular Localization||Cell membrane; Single-pass type I membraneprotein.|
|Uniprot ID||CSF1R: P07333
|Alternative Names||Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; M-CSF-R; 22.214.171.124; Proto-oncogene c-Fms; CD115; CSF1R; FMS;|
|Research Areas||cardiovascular|angiogenesis|cytokines|immunology|innate immunity|macrophage / inflamm.|csfs|signal transduction|protein phosphorylation|ser / thr kinases kinases||
Background for Macrophage colony-stimulating factor 1 receptor
Human CSF1R/M-CSFR PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human CSF1R/M-CSFR PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 25-30min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.