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SKU:EZ0741
Pack Size:For 5 plates, 96 wells each
Application:ELISA
Sensitivity:<10pg/ml
Sample Type:cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Price: $215.00
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Product Features:

Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

Overview

Product Name Human CXCL16 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
SKU/Catalog Number EZ0741
Description A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CXCL16 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Cite This Product Human CXCL16 EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0741)
Replacement Item This antibody may replace the following items: sc-27343|sc-27342|sc-376395|sc-27344|sc-398792|sc-514363 from Santa Cruz Biotechnology.
Validated Species Human
Application ELISA

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Immunogen Expression system for standard: E.coli; Immunogen sequence: N49-P137
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Pack Size For 5 plates, 96 wells each

Properties

Sensitivity <10pg/ml
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range 93.7pg/ml-6000pg/ml
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Capture Antibody monoclonal antibody from mouse
Detection Antibody polyclonal antibody from goat
Natural Levels Some research articles suggesting the natural levels of CXCL16 are listed below:
Storage Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Kit Components

Catalog numberDescriptionQuantity
EZ0741-CACapture Antibody For CXCL16500ul
EZ0741-DABiotinylated Detection Antibody For CXCL16500ul
ST0000-10lyophilized recombinant human Lipocalin-2/CXCL16 standard10ng/tubex2
AR1103Avidin-Biotin-Peroxidase Complex(ABC)500ul(dilution 1:100)

Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.

*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.

See Prices For Extra Components

Material Required But Not Provided

Components Included In The EZ Set Accessory Kit (EZA001)

  • Antibody diluent buffer
  • ABC diluent buffer
  • Sample diluent buffer
  • TMB
  • TMB stop solution
  • 5x 96 well plates, plate sealers

*The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other sources if it is of convenience to you.

Components And Equipment Not Included In The EZ Set Accessory Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
*Reagents needed for sample collection and preparation are not included.

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CXCL16
Protein Name C-X-C motif chemokine 16
Molecular Weight 27579 MW
Protein Function Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. .
Tissue Specificity Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.
Sequence Similarities Belongs to the intercrine alpha (chemokine CxC) family.
Subcellular Localization Cell membrane ; Single-pass type I membrane protein . Secreted. Also exists as a soluble form.
Uniprot ID Q9H2A7
Alternative Names C-X-C motif chemokine 16;Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein;SR-PSOX;Small-inducible cytokine B16;Transmembrane chemokine CXCL16;CXCL16;SCYB16, SRPSOX;UNQ2759/PRO6714;
Research Areas |immunology|innate immunity|macrophage / inflamm.| immunology|chemokines|alpha chemokines (cxc)|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for C-X-C motif chemokine 16

Chemokine(C-X-C motif) ligand 16(CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines(with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T(NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16(CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

Human CXCL16 EZ-Set™ ELISA Kit (DIY Antibody Pairs) Images

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Human CXCL16 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
Human CXCL16 EZ Set ELISA Kit standard curve

Intra/Inter Assay Precision

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Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n161616242424
Mean(ng/ml)1.82.74.11.92.73.9
Standard deviation0.0850.170.140.120.210.22
CV(%)4.76.23.36.17.85.6

Typical Data Obtained from Human CXCL16 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

(TMB reaction incubate at 37°C for 25-30min)

Concentration(pg/ml)093.7187.5375750150030006000
O.D.0.0550.2040.3580.7870.9061.6862.0292.120

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

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