Human CXCL4/PF4 ELISA Kit PicoKine®

CXCL4/PF4 ELISA kit for Human

Human CXCL4/PF4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0726
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human CXCL4/PF4 ELISA Kit PicoKine®

View all CXCL4/PF4 ELISA kits

SKU/Catalog Number

EK0726

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CXCL4/PF4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CXCL4/PF4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0726)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E32-S101

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0726 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0726 is reactive to PF4 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0726 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CXCL4/PF4

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Kit Components

Catalog Number Description Quantity
EK0726-CAP Anti-Human PF4 Pre-coated 96-well strip microplate 1
EK0726-ST Human PF4 Standard 2 vials, 10 ng/tube
EK0726-DA Human PF4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0810.1270.1960.2640.3810.6611.2921.991

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CXCL4/PF4, Dilution ratio of 1:2000, concentration in serum is 810-20600 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CXCL4/PF4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3551700479833715475118
Standard deviation24.8579.9249.4924.2691.27348.02
CV (%)7%4.7%5.2%7.2%5.9%6.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CXCL4/PF4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135530235331633123.046.9%
Sample 21700179018231661174365.533.7%
Sample 347984682461644464635127.332.7%
*number of samples for each test n=16.

Gene/Protein Information For PF4 (Source: Uniprot.Org, NCBI)

Gene Name

PF4

Full Name

Platelet factor 4

Weight

10.845kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A PF4 CXCL4, PF-4, SCYB4 platelet factor 4 platelet factor 4|C-X-C motif chemokine 4|chemokine (C-X-C motif) ligand 4|iroplact|oncostatin-A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PF4, check out the PF4 Infographic

PF4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PF4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0726 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CXCL4 promoted the production of CD4+CD25+FOXP3+treg cells in mouse sepsis model through regulating STAT5/FOXP3 pathway

Platelet-derived alpha-granules are associated with inflammation in patients with NK/T-cell lymphoma-associated hemophagocytic syndrome

Have you used Human CXCL4/PF4 ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human CXCL4/PF4 ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

11 Customer Q&As for Human CXCL4/PF4 ELISA Kit PicoKine®

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

L. Thompson

Verified customer

Asked: 2020-10-21

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-10-21

Question

I want to measure PF4 in human bone marrow aspirate plasma. Is your product EK0726 has been used on bone marrow before? Do you have any recommendation for a suitable starting dilutions?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

Unfortunately, the product EK0726 has not been tested with bone marrow aspirate before. However, if the bone marrow aspirate cell plasma is prepared cell free and clear as a liquid we believed that it will work with this sample types. For the dilutions, we recommend a starting dilution of 1:200 to 1:2000 for this kind of samples, please remember that this recommendation should only be used as a starting point and the optimal dilution should be determined by you based on researched literature and your knowledge about this samples.

Boster Scientific Support

Answered: 2020-02-24

Question

To Technical Support: Kindly advise if your product Human CXCL4/PF4 ELISA Kit PicoKine is Anti-Heparin complex or not.

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

The product Human CXCL4/PF4 ELISA Kit PicoKine has not been tested if it will also recognize the bound PF4-heparin complex form. The information we have is that this antibody pair is binding on the PF4 protein somewhere between aa 32-101, thus to determine whether this kit will also detect the bound PF4-heparin complex form would need to be determined by the investigator.

Boster Scientific Support

Answered: 2019-11-28

Question

Q: can I use citrate plasma as samples in Human CXCL4 Picokine® ELISA Kit (Catalog # EK0726)?

W. Phillips

Verified customer

Asked: 2019-02-15

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CXCL4 causing disruption of its protein structure. CXCL4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CXCL4 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-02-15

Question

Q: how do I analyze ELISA data? I have performed CXCL4 level in plasma.

B. Miller

Verified customer

Asked: 2018-08-09

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-08-09

Question

Q: how to thaw whole blood sample for CXCL4 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-06-01

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL4 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-06-01

Question

Q: if the enzyme conjugated CXCL4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL4 antigen?

M. Wright

Verified customer

Asked: 2018-03-15

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-03-15

Question

Q: Can CXCL4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

M. King

Verified customer

Asked: 2018-01-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-01-23

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CXCL4 using the EK0726 Human CXCL4 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-05-09

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2016-05-09

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-05-02

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-05-02

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

L. Clark

Verified customer

Asked: 2016-01-12

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-01-12

$499
In stock, 1 left.

Order within -6 hours and -32 minutes to receive by Fri Mar 29

Get A Quote
Eddy test
In stock
Order Product
EK0726
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.