Human CYR61/CCN1 ELISA Kit PicoKine®

Cyr61/CCN1 ELISA kit for Human

Human CYR61/CCN1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCN1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1203
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Product Name

Human CYR61/CCN1 ELISA Kit PicoKine®

View all Cyr61/CCN1 ELISA kits

SKU/Catalog Number

EK1203

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CYR61/CCN1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCN1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CYR61/CCN1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1203)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: A22-D381

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1203 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1203 is reactive to CCN1 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Application Guarantee

EK1203 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Cyr61/CCN1

Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.

Kit Components

Catalog Number Description Quantity
EK1203-CAP Anti-Human CCN1 Pre-coated 96-well strip microplate 1
EK1203-ST Human CCN1 Standard 2 vials, 10 ng/tube
EK1203-DA Human CCN1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0060.0610.1370.2140.3750.7221.0971.834

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cyr61/CCN1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CYR61/CCN1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6124881459242806
Standard deviation2.8616.1255.353.317.6664.48
CV (%)4.7%6.5%6.8%5.6%7.3%8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cyr61/CCN1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 161646358612.293.7%
Sample 22482732632552599.313.5%
Sample 381490778386984347.995.6%
*number of samples for each test n=16.

Gene/Protein Information For CCN1 (Source: Uniprot.Org, NCBI)

Gene Name

CCN1

Full Name

CCN family member 1

Weight

42.027kDa

Superfamily

CCN family

Alternative Names

CCN family member 1; CCN1; CCN1IGF-binding protein 10; Cyr61; Cysteine-rich angiogenic inducer 61; cysteine-rich, angiogenic inducer, 61; GIG1; GIG1cysteine-rich heparin-binding protein 61; IBP-10; IGFBP-10; IGFBP10cysteine-rich, anigogenic inducer, 61; Insulin-like growth factor-binding protein 10; protein CYR61; Protein GIG1 CCN1 CYR61, GIG1, IGFBP10 cellular communication network factor 1 CCN family member 1|IBP-10|IGF-binding protein 10|IGFBP-10|cysteine rich angiogenic inducer 61|cysteine-rich heparin-binding protein 61|cysteine-rich, anigogenic inducer, 61|insulin-like growth factor-binding protein 10|protein CYR61

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCN1, check out the CCN1 Infographic

CCN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1203 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Novel treatment avenues for uterine leiomyoma: a new implication for endothelin?

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6 Customer Q&As for Human CYR61/CCN1 ELISA Kit PicoKine®

Question

Q: Can CYR61 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

G. Chen

Verified customer

Asked: 2020-06-12

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-06-12

Question

Q: can you recommend the dilution ratio of serum samples for detection of CYR61 in Human human milk? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-01-18

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CYR61 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-01-18

Question

Q: how can I thaw whole blood sample for CYR61 ELISA after freezing?

H. Lee

Verified customer

Asked: 2019-03-19

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CYR61 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-03-19

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-12-14

Question

Hi, I want to buy this antibody EK1203 Human CYR61/CCN1 ELISA Kit to blocking protein in vivo for some mice experiment. Can you recommend a working concentration for this antibody to use in vivo? Thanks!

Verified Customer

Verified customer

Asked: 2018-05-09

Answer

Thank you for your query. Unfortunately our products are not intended for in vivo use, so we don't have any recommendations for you to use EK1203 Human CYR61/CCN1 ELISA Kit on this kind of experiment. This antibody has only been tested in Western blot and immunohistochemistry, we are not sure if it will successfully block this protein in vivo. The buffer contains 0.02% sodium azide, which may be toxic to the animals, so I would suggest removing the azide,if you do decide to proceed and use this antibody EK1203 Human CYR61/CCN1 ELISA Kit in vivo.

Boster Scientific Support

Answered: 2018-05-09

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

D. Roberts

Verified customer

Asked: 2016-01-23

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-01-23

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