Human Cystatin C EZ-Set™ ELISA Kit (DIY Antibody Pairs)

SKU:EZ0678
Pack Size:For 5 plates, 96 wells each
Validated Species:Human
Application:ELISA
Sensitivity:<10pg/ml
Assay Range:312pg/ml-20000pg/ml
Sample Type:cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.
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Product Features:

Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

Overview

Product Name Human Cystatin C EZ-Set™ ELISA Kit (DIY Antibody Pairs)
SKU/Catalog Number EZ0678
Description A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Cystatin C in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Cite This Product Human Cystatin C EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0678)
Replacement Item This antibody may replace the following items: from Santa Cruz Biotechnology.
Host
Validated Species 194
Predicted Species

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application 18

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen Expression system for standard: NSO, M1-A146
Conjugate
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Pack Size For 5 plates, 96 wells each

Properties

Clonality
Clone Number
Sensitivity <10pg/ml
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range 312pg/ml-20000pg/ml
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.

*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Capture Antibody
Detection Antibody
Normal Levels Some research articles suggesting the natural levels of CST3 are listed below:
Form
Contents
Concentration
Activating Reagent
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Purification
Isotype

Kit Components

Catalog numberDescriptionQuantityBuffersEZ0678-CARabbit anti- human Cystatin C polyclonal antibody(Capture Antibody)500 μL, 0.4mg/mL(recommended dilution 1:100)0.5% Na2HPO4, 0.02% Sodium azide and 50% GlycerolEZ0678-DABiotinylated goat anti- human Cystatin C polyclonal antibody(Detection Antibody)500 μL, 2.3μg/mL(recommended dilution 1:100)0.02% Sodium azide, 0.5%BSA, 0.2%Tris, 1%NaCl and 30% GlycerolEZ0678-STLyophilized recombinant human Cystatin C standard20ng/tubex2AR1103Avidin-Biotin-Peroxidase Complex(ABC)500ul(recommended dilution 1:100)<

*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.

See Prices For Extra Components

Material Required But Not Provided

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers
  • 7. Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 ?m filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (neutral PBS or TBS).
    Preparation of 0.01M TBS: Add 1.2 g Tris, 8.5 g Nacl; 450 ?L of purified acetic acid or 700 ?L of concentrated hydrochloric acid to 1000 mL H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1 L.
    Preparation of 0.01M PBS: Add 8.5 g sodium chloride, 1.4 g Na2HPO4 and 0.2 g NaH2PO4 to 1000 mL distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*The EZ Set Accessory Kit (EZA001) provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you.

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CST3
Protein Name
Molecular Weight
Protein Function As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Tissue Specificity Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
Sequence Similarities
Subcellular Localization Secreted
Uniprot ID P01034
Alternative Names ARMD11; bA218C14.4(cystatin C); Cst3; Cystatin-3; Cystatin-C; CYTC_HUMAN; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin;
Reseach Areas 2327,2337,2338,2513,2330,2421,2331,2506,2381,2367,2432|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for CST3

Cystatin C or cystatin 3(formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight(approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus(cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2(enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3(it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Human Cystatin C EZ-Set™ ELISA Kit (DIY Antibody Pairs) Images

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Human Cystatin C EZ-Set™ ELISA Kit (DIY Antibody Pairs)
Human Cystatin C EZ-Set ELISA Kit standard curve

Intra/Inter Assay Precision

|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|687|2837|10374|647|2715|10112 Standard deviation|43.96|207.1|684.68|51.11|217.2|859.52 CV(%)|6.4%|7.3%|6.6%|7.9%|8%|8.5%

Typical Data Obtained from Human Cystatin C EZ-Set™ ELISA Kit (DIY Antibody Pairs)

(TMB reaction incubate at 37℃ for 15-20min)

Concentration(pg/ml)03126251250250050001000020000
O.D.0.0620.1430.2120.3470.5820.9921.4441.836

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Cystatin C in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
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EZ0678
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