Human DEFA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human DEFA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human DEFA1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ1514
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human DEFA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all alpha-Defensin 1 products

SKU/Catalog Number

EZ1514

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human DEFA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human DEFA1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human DEFA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1514)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D39-C94

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1514 is reactive to DEFA1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EZ1514 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ1514-CA Rabbit anti- human DEFA1 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ1514-DA Biotinylated goat anti- human DEFA1 polyclonal antibody (Detection Antibody) 500 µl, 5 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ1514-ST Lyophilized recombinant human DEFA1 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0910.1390.1720.2030.3530.6331.2772.163

Data Example Images

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4621682949663518979984
Standard deviation21.7189.15531.7842.5596.75479.23
CV (%)4.7%5.3%5.6%6.7%5.1%4.8%

Gene/Protein Information For DEFA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P59665

Gene ID

1667

Gene Name

DEFA1

Full Name

Neutrophil defensin 1

Weight

10.201kDa

Superfamily

alpha-defensin family

Alternative Names

alpha 2; alphaDefensin 1; alpha-Defensin 1; DEF1; DEF1DEFA1B; DEFA1; DEFA2; defensin, alpha 1, myeloid-related sequence; defensin, alpha 1HP-1; HNP-1; HNP-1HP1; HP1; MRS; MRSMGC138393; myeloid-related sequence; neutrophil defensin 1 DEFA1 DEF1, DEFA2, HNP-1, HP-1, HP1, MRS defensin alpha 1 neutrophil defensin 1|defensin, alpha 1, myeloid-related sequence|defensin, alpha 2|myeloid-related sequence

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DEFA1, check out the DEFA1 Infographic

DEFA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DEFA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EZ1514

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human DEFA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human DEFA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Human DEFA1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

$255

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EZ1514
$255.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.