Human DLL1 ELISA Kit PicoKine®

Dll1 ELISA kit for Human

Human DLL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human DLL1 in cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1276
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk.

Product Name

Human DLL1 ELISA Kit PicoKine®

View all Dll1 ELISA kits

SKU/Catalog Number

EK1276

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human DLL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human DLL1 in cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human DLL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1276)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-G540

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1276 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1276 is reactive to Dll1 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk.

Application Guarantee

EK1276 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Dll1

DLL1 (DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Kit Components

Catalog Number Description Quantity
EK1276-CAP Anti-Human Dll1 Pre-coated 96-well strip microplate 1
EK1276-ST Human Dll1 Standard 2 vials, 10 ng/tube
EK1276-DA Human Dll1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0030.2320.3730.7051.0121.4591.8111.902

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Dll1, Dilution ratio of 1:5, concentration in serum is 830-2162 pg/ml..

Some articles we found to cite concentrations of Dll1 in samples: 21426637 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human DLL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10563423531026622503
Standard deviation4.6227.2694.124.6931.11137.66
CV (%)4.4%4.3%4%4.6%4.7%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Dll1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1105104941061024.814.7%
Sample 263457859559660020.483.4%
Sample 32353220224592342233991.343.9%
*number of samples for each test n=16.

Gene/Protein Information For Dll1 (Source: Uniprot.Org, NCBI)

Gene Name

Dll1

Full Name

Delta-like protein 1

Weight

78.449kDa

Alternative Names

delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1 Dll1|Delt, Delta1|delta like canonical Notch ligand 1|delta-like protein 1|delta like-1|delta-like 1|drosophila Delta homolog 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Dll1, check out the Dll1 Infographic

Dll1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Dll1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Human DLL1 ELISA Kit PicoKine®

Question

Q: how do I analyze ELISA data? I measured Dll1 level in serum.

A. Adams

Verified customer

Asked: 2020-08-10

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-08-10

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

P. Miller

Verified customer

Asked: 2019-09-22

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-09-22

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-06-16

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-06-16

Question

Q: What is the optimal O.D. value for Dll1 ELISA kit? I performed your Dll1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Dll1 even though the O.D. values are not very high?

W. Babu

Verified customer

Asked: 2018-05-29

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Dll1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Dll1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-05-29

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Dll1 using the EK1276 Human Dll1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-03-24

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-03-24

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

S. Banerjee

Verified customer

Asked: 2014-09-01

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-09-01

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