Human Fetuin B/FETUB ELISA Kit PicoKine®

FETUB ELISA kit for Human

Human Fetuin B ELISA Kit PicoKine™ (96 Tests). Quantitate Human FETUB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1498
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Fetuin B/FETUB ELISA Kit PicoKine®

View all FETUB ELISA kits

SKU/Catalog Number

EK1498

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Fetuin B ELISA Kit PicoKine™ (96 Tests). Quantitate Human FETUB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Fetuin B/FETUB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1498)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: C16-P382

Sensitivity

<12 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1498 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1498 is reactive to FETUB in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1498 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of FETUB

Fetuin-B is a protein that in humans is encoded by the FETUB gene. It is mapped to 3q27, where the FETUA gene is localized. The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells.

Kit Components

Catalog Number Description Quantity
EK1498-CAP Anti-Human FETUB Pre-coated 96-well strip microplate 1
EK1498-ST Human FETUB Standard 2 vials, 10 ng/tube
EK1498-DA Human FETUB Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0030.0350.0620.1130.2050.4580.8281.843

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect FETUB, Dilution ratio of 1:2000, concentration in serum and plasma is around 2 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Fetuin B/FETUB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2341109454924811554773
Standard deviation17.0858.77209.2521.3264.68229.1
CV (%)7.3%5.3%4.6%8.6%5.6%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FETUB in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 123424521222522912.15.2%
Sample 21109125811411184117355.824.7%
Sample 345494286476946924574184.064%
*number of samples for each test n=16.

Gene/Protein Information For FETUB (Source: Uniprot.Org, NCBI)

Gene Name

FETUB

Full Name

Fetuin-B

Weight

42.055kDa

Superfamily

fetuin family

Alternative Names

FETUB; Fetuin B FETUB 16G2, Gugu, IRL685 fetuin B fetuin-B|fetuin-like protein IRL685

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FETUB, check out the FETUB Infographic

FETUB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FETUB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1498 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Increased levels of the novel hepatokine fetuin B in patients with intrahepatic cholestasis of pregnancy

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10 Customer Q&As for Human Fetuin B/FETUB ELISA Kit PicoKine®

Question

Does EK1498 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Human Fetuin B/FETUB ELISA Kit PicoKine® (EK1498) doesn't contain native human biological materials. doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Q: can I use citrate plasma as samples in Human FETUB Picokine® ELISA Kit (Catalog # EK1498)?

Verified Customer

Verified customer

Asked: 2020-10-10

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of FETUB causing disruption of its protein structure. FETUB may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FETUB ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-10-10

Question

Q: if the enzyme conjugated FETUB antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FETUB antigen?

Verified Customer

Verified customer

Asked: 2020-03-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-03-14

Question

Q: What is the optimal O.D. value for FETUB ELISA kit? I used your FETUB ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain FETUB even though the O.D. values are not very high?

L. Sunder

Verified customer

Asked: 2019-04-26

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FETUB ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of FETUB that can be declared/interpreted as positive by the above standard is 12pg/ml.

Boster Scientific Support

Answered: 2019-04-26

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-03-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-03-17

Question

Q: how to analyze ELISA data? I have performed FETUB level in cell lysates.

C. Chakrabarti

Verified customer

Asked: 2018-06-20

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-06-20

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring FETUB using the EK1498 Human FETUB Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-05-05

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-05-05

Question

Q: can you recommend the dilution ratio of serum samples for detection of FETUB in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-04-13

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FETUB ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-04-13

Question

Q: which procedure should I follow in order to thaw whole blood sample for FETUB ELISA after freezing?

L. Zhao

Verified customer

Asked: 2017-08-02

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FETUB for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2017-08-02

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

T. Puri

Verified customer

Asked: 2017-05-19

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-05-19

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