Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

FGF-19 ELISA antibody pairs, Human, for 5 plates

Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FGF19 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Product Info Summary

SKU: EZ1160
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all FGF-19 products

SKU/Catalog Number

EZ1160

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FGF19 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1160)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L25-K216

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1160 is reactive to FGF19 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ1160 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ1160-CA Rabbit anti- human FGF19 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ1160-DA Biotinylated goat anti- human FGF19 polyclonal antibody (Detection Antibody) 500 µl, 2.5 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EK1160-ST Lyophilized recombinant human FGF19 standard 10 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Incubator.
  • 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 5. Clean tubes and Eppendorf tubes.
  • 6. 96 well microplate (Cat# AR1100)
  • 7. Plate Sealers.
  • 8. Capture Antibody Diluent: PBS.
  • 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 12. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0290.0980.1570.2580.5741.1941.732.067

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)103396669152388709
Standard deviation6.691725.410.9420.9534.74
CV (%)6.5%4.3%3.8%7.2%5.4%4.9%

Gene/Protein Information For FGF19 (Source: Uniprot.Org, NCBI)

Gene Name

FGF19

Full Name

Fibroblast growth factor 19

Weight

24.003kDa

Superfamily

heparin-binding growth factors family

Alternative Names

FGF19; FGF-19; fibroblast growth factor 19 FGF19 fibroblast growth factor 19 fibroblast growth factor 19|FGF-19

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FGF19, check out the FGF19 Infographic

FGF19 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FGF19: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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7 Customer Q&As for Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

What is the recommended sample diluent for EZ0767,EZ0994 and EZ1160?

Verified customer

Asked: 2019-05-09

Answer

The recommended sample diluent of the Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0767), Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0994) and Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ1160) for serum is 1:1.

Boster Scientific Support

Answered: 2019-05-10

Question

Do you recommend to include 0.05% Tween 20 v/v in the wash buffer and do you foresee any issue?

Verified customer

Asked: 2019-05-09

Answer

For the Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0767), Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0994) and Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ1160), you can include 0.05% Tween 20 v/v in the wash buffer. It is OK to use 1x PBS with NaCl, KCl, Na2HPO4 and KH2PO4.

Boster Scientific Support

Answered: 2019-05-10

Question

Is washing recommended after coating (before blocking) for EZ0767, EZ0994 and EZ1160?

Verified customer

Asked: 2019-05-09

Answer

For the Human GDF-15 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0767), Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0994) and Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ1160), washing or not washing would not make too much difference to the assay result.

Boster Scientific Support

Answered: 2019-05-10

Question

Is washing recommended after coating (before blocking) for EZ1160?

Verified customer

Asked: 2019-05-09

Answer

For the Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ1160), washing or not washing would not make too much difference to the assay result.

Boster Scientific Support

Answered: 2019-05-10

Question

Do you recommend washing step after sample/standard/control incubation in the assay procedure for EZ0767, EZ0994 and EZ1160?

Verified customer

Asked: 2019-05-09

Answer

No, we do not recommend washing step after sample/standard/control incubation for EZ0767, EZ0994 and EZ1160.

Boster Scientific Support

Answered: 2019-05-10

Question

Would you recommend using PBS pH7.2 – 7.4 or Sodium Bicarb buffer pH 9.6 for EZ0767, EZ0994 and EZ1160?

Verified customer

Asked: 2019-05-09

Answer

Both PBS pH7.2 – 7.4 and Sodium Bicarb buffer pH 9.6 are commonly used capture antibody diluent. Our lab hasn't validated their performance. If you prefer, please run a pilot test to validate their effect. We recommend using "Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4", which is stated on our datasheet.

Boster Scientific Support

Answered: 2019-05-10

Question

Is there pH requirement for “Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4”?

Verified customer

Asked: 2019-05-07

Answer

The pH value should be 8.0-9.0.

Boster Scientific Support

Answered: 2019-05-07

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