Human Ficolin-3 PicoKine™ Fast ELISA Kit


SKU FEK1259
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, serum and plasma(heparin).
Sample Volume 100ul per well
Sensitivity <20pg/ml
Assay Range 0.78ng/ml-50ng/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Human Ficolin-3 PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK1259
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Ficolin-3/FCN3 with <20pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Ficolin-3/FCN3 tissue specificity: Liver and lung. In liver it is produced by bile duct epithelial cells and hepatocytes. In lung it is produced by both ciliated bronchial epithelial cells and type II alveolar epithelial cells. .
Cite This Product Human Ficolin-3 PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1259)
Sample Type cell culture supernates, serum and plasma(heparin). Anticoagulant(s): heparin
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <20pg/ml
Assay Range 0.78ng/ml-50ng/ml
Immunogen Expression system for standard: NSO; Immunogen sequence: Q24-R299
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
FEK1259-CAP 96-well plate precoated with anti-Human FCN3 antibody 1
FEK1259-ST lyophilized recombinant Human FCN3 standard 50ng/tube
FEK1259-DA biotinylated anti-Human FCN3 antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human Ficolin-3 PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 25-30min)

Concentration(ng/ml)00.781.563.126.2512.52550
O.D.0.1590.1880.2390.3230.4940.8171.2641.915

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)12165741264801219625325739
Standard deviation80.25396.121403.4480.45450.211853.2
CV(%)6.6%6.9%5.3%6.6%7.2%7.2%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11216134712671181125262.414.9%
Sample 257415533587664845908354.015.9%
Sample 326480265662652922150254311894.677.4%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O75636
Gene Name FCN3
Protein Name Ficolin-3
Tissue Specificity Liver and lung. In liver it is produced by bile duct epithelial cells and hepatocytes. In lung it is produced by both ciliated bronchial epithelial cells and type II alveolar epithelial cells. .
Alternative Names Ficolin-3;Collagen/fibrinogen domain-containing lectin 3 p35;Collagen/fibrinogen domain-containing protein 3;Hakata antigen;FCN3;FCNH, HAKA1;
Subcellular Localization Secreted. Found in blood plasma, bronchus, alveolus and bile duct.
Molecular Weight 32903 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota. .
Research Areas Human

*You can search these to find other products in these research areas.
Background Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin(FN1), elastin(ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

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FEK1259
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$419.00

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