Human Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Galectin-1 ELISA antibody pairs, Human, for 5 plates

Human Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human LGALS1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). Cited in 3 publication(s).

Product Info Summary

SKU: EZ0762
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all Galectin-1 products

SKU/Catalog Number

EZ0762

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human LGALS1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Galectin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0762)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-D135

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0762 is reactive to LGALS1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ0762 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ0762-CA Rabbit anti- human LGALS1 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0762-DA Biotinylated goat anti- human LGALS1 polyclonal antibody (Detection Antibody) 500 µl, 5 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EK0762-ST Lyophilized recombinant human LGALS1 standard 10 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Incubator.
  • 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 5. Clean tubes and Eppendorf tubes.
  • 6. 96 well microplate (Cat# AR1100)
  • 7. Plate Sealers.
  • 8. Capture Antibody Diluent: PBS.
  • 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 12. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0080.0770.1560.2950.5260.9621.5642.104

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7252786782485730148245
Standard deviation36.25114.23485.0954.85177.83610.13
CV (%)5%4.1%6.2%6.4%5.9%7.4%

Gene/Protein Information For LGALS1 (Source: Uniprot.Org, NCBI)

Gene Name

LGALS1

Full Name

Galectin-1

Weight

14.716kDa

Alternative Names

14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LGALS1, check out the LGALS1 Infographic

LGALS1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LGALS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EZ0762 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of immune checkpoint molecules in endometrial carcinoma

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma

Tang D, Yuan Z, Xue X, Lu Z, Zhang Y, Wang H, Chen M, An Y, Wei J, Zhu Y, Miao Y, Jiang K. Int J Cancer. 2012 May 15;130(10):2337-48. Doi: 10.1002/Ijc.26290. Epub 2011 Nov 28. High Expression Of Galectin-1 In Pancreatic Stellate Cells Plays A Role...

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