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SKU:EZ0767
Pack Size:For 5 plates, 96 wells each
Validated Species:Human
Host:No
Application:ELISA
Sensitivity:<10pg/ml
Assay Range:31.2pg/ml-2000pg/ml
Sample Type:cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
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Product Features:

Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

Overview

Product Name Human GDF-15 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
SKU/Catalog Number EZ0767
Description A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects GDF-15 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Cite This Product Human GDF-15 EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0767)
Validated Species Human
Application ELISA

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Immunogen Expression system for standard: CHO, A197-I308
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Pack Size For 5 plates, 96 wells each

Properties

Sensitivity <10pg/ml
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range 31.2pg/ml-2000pg/ml
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Kit Components

Catalog numberDescriptionQuantityBuffers
EZ0767-CARabbit anti- human GDF-15 polyclonal antibody(Capture Antibody)500 μL, 0.4mg/mL(recommended dilution 1:100)0.5% Na2HPO4, 0.02% Sodium azide and 50% Glycerol
EZ0767-DABiotinylated goat anti- human GDF-15 polyclonal antibody(Detection Antibody)500 μL, 1μg/mL(recommended dilution 1:100)0.02% Sodium azide, 0.5%BSA, 0.2%Tris, 1%NaCl and 30% Glycerol
EZ0767-STLyophilized recombinant human GDF-15 standard10ng/tubex2
AR1103Avidin-Biotin-Peroxidase Complex(ABC)500ul(recommended dilution 1:100)<

*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.

See Prices For Extra Components

Material Required But Not Provided

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers
  • 7. Capture Antibody Diluent: 100 mL H2O, 0.5 g Na2HPO4.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 ?m filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (neutral PBS or TBS).
    Preparation of 0.01M TBS: Add 1.2 g Tris, 8.5 g Nacl; 450 ?L of purified acetic acid or 700 ?L of concentrated hydrochloric acid to 1000 mL H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1 L.
    Preparation of 0.01M PBS: Add 8.5 g sodium chloride, 1.4 g Na2HPO4 and 0.2 g NaH2PO4 to 1000 mL distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*The EZ Set Accessory Kit (EZA001) provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you.

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name GDF15
Protein Function Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling (By similarity).
Tissue Specificity Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. Detected in plasma (at protein level).
Uniprot ID Q99988
Alternative Names Growth/differentiation factor 15; GDF-15; Macrophage inhibitory cytokine 1; MIC-1; NSAID-activated gene 1 protein; NAG-1; NSAID-regulated gene 1 protein; NRG-1; Placental TGF-beta; Placental bone morphogenetic protein; Prostate differentiation factor; GDF15; MIC1; PDF; PLAB; PTGFB;
Reseach Areas Cardiovascular, Cell Biology, Cell Cycle, Cell Division, Cytokinesis, Cytoskeleton / Ecm, Extracellular Matrix, Growth Factors/Hormones, Hypertrophy, Signal Transduction, Structures|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for GDF15

GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

Human GDF-15 EZ-Set™ ELISA Kit (DIY Antibody Pairs) Images

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Human GDF-15 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
Human GDF-15 EZ-Set ELISA Kit standard curve

Intra/Inter Assay Precision

|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|53|252|1068|53|266|1115 Standard deviation|3.55|16.12|74.76|3.92|20.21|81.39 CV(%)|6.7%|6.4%|7%|7.4%|7.6%|7.3%

Typical Data Obtained from Human GDF-15 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

(TMB reaction incubate at 37°C for 20-25min)

Concentration(pg/ml)031.262.512525050010002000
O.D.0.0020.1060.2260.4490.7941.3571.9972.479

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

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