Human High Sensitivity (4-plex) Sample Demo Kit

ELISA kit for Multiplex detection

Human High Sensitivity (4-plex) Sample Kit is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 4 biomarkers or analytes. Attention: this product requires a special plate reader. Please make sure you have the proper equipment before ordering. For details please contact [email protected]

Product Info Summary

Species: Human
Sample Type: Serum, EDTA Plasma
Volume Required per Well: Min 25 µl
Assay Length: 2.25 hours
Specificity: All analytes < 1% intra-panel cross-reactivity
Assay Type: Planar Based Multiplexed ELISA
Detection Method: Chemiluminescent
Multiplex Format: 96-well solid plate
Catalog: MEK2002

Product Overview

Human High Sensitivity (4-plex) Sample Kit is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 4 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 4 markers in the panel within 2.25 hours. The Human High Sensitivity (4-plex) Sample Kit provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

*For a limited time, we are offering this kit on trial basis for only $99 per kit. Limit to 1 kit per lab. Claim yours today by contacting us at [email protected] for details.

Assay Ranges

Calibrator Range Units
IL-4 2000-2.74 pg/ml
IL-6 3000-4.11 pg/ml
IL-10 3000-4.11 pg/ml
IFN-γ 8000-10.97 pg/ml

Kit Components

Each kit contains a 96-well plate (not strip well), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.

Reagents Include:

Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips

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The multiplex ELISA kits require special plate readers. For more info see special plate reader. The $800 demo option is for new customer only, see more info at demo options.



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