Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-10 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC. Cited in 8 publication(s).
Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Info At A Glance
|Size:||For 5 plates, 96 wells each, reagents only, plates are not included|
|Sample Type:||cell culture supernates, serum and plasma( heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
|Product Name||Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all IL-10 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-10 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.|
|Cite This Product||Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0416)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma( heparin, EDTA, citrate).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: S19-N178|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||For 5 plates, 96 wells each, reagents only, plates are not included|
Gene/Protein Basic Information For IL10 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3586|
|Species Of This Entry||Human|
|Alternative Names||IL-10|CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF|
|Post Tranlational Modifications||Phosphorylation; Methylation; Acetylation; Cleavage; Oxidation; Demethylation; Glycosylation; Citrullination; Dephosphorylation; Nitration; Nitrosylation; Ubiquitination; Iodination; Reduction; Deacetylation|
|Gene Location||1q32.1, on Chromasome 1, gene sequence: NC_000001.11 (206767602..206772494, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL10 (Source: Uniprot.org, NCBI)
|Protein Function||Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.|
|Tissue Specificity||Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.|
|Interacting Proteins||IL10RA; IL10RB|
|Related Diseases||Inflammation; Malignant Neoplasms; Autoimmune Reaction; Inflammatory Response; Neoplasms; Colitis; Autoimmune Diseases; Arthritis; Infective Disorder; Inflammatory Bowel Diseases; Lymphoma; Rheumatoid Arthritis; Intestinal Diseases; Asthma; Sclerosis; Lupus Erythematosus, Systemic; Hepatitis; Diabetes Mellitus; Hiv Infections; Multiple Sclerosis|
|Related Pathways||Immune Response; Secretion; Pathogenesis; Cytokine Production; Inflammatory Response; Cytokine Secretion; Cell Proliferation; Cell Activation; Innate Immune Response; Cell Death; T Cell Proliferation; T Cell Activation; Hypersensitivity; Sensitization; Lymphocyte Proliferation; Cell Cycle; Macrophage Activation; Phagocytosis; Angiogenesis; Methylation|
|Background||Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.|
|Scientific References||PMID: 1847510 by Vieira P., et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.|
PMID: 9405662 by Gesser B., et al. Identification of functional domains on human interleukin 10.
Kit Components And Assay QC Data Details Of Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EZ0416-CAP||96-well plate precoated with anti-Human IL-10 antibody||1|
|EZ0416-ST||lyophilized recombinant Human IL-10 standard||10ng/tube|
|EZ0416-DA||biotinylated anti-Human IL-10 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EZ0416 Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 8
|Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...||PubMed ID 23423691|
|Berni Canani R, Paparo L, Nocerino R, Cosenza L, Pezzella V, Di Costanzo M, Capasso M, Del Monaco V, D'Argenio V, Greco L, Salvatore F. Clin Epigenetics. 2015 Mar 31;7(1):38. Doi: 10.1186/S13148-015-0070-8. Ecollection 2015. Differences In Dna Met...||PubMed ID 25859290|
|Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells||PubMed ID 26295043|
|Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica||PubMed ID 26630909|
|The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility||PubMed ID 29914245|
|Increased Age-Related Cardiac Dysfunction in Bradykinin B2 Receptor%u2013Deficient Mice||PubMed ID 25452401|
|Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome||PubMed ID 29062341|
|Alterations of serum cytokine levels and their relation with inflammatory markers in candidemia||PubMed ID 25627661|
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