Human IL-18BP ELISA Kit PicoKine®

IL18BP ELISA kit for Human

Human IL-18BP ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL18BP in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1282
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human IL-18BP ELISA Kit PicoKine®

View all IL18BP ELISA kits

SKU/Catalog Number

EK1282

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-18BP ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL18BP in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-18BP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1282)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T31-G194

Sensitivity

<10 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1282 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1282 is reactive to IL18BP in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK1282 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL18BP

Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.

Kit Components

Catalog Number Description Quantity
EK1282-CAP Anti-Human IL18BP Pre-coated 96-well strip microplate 1
EK1282-ST Human IL18BP Standard 2 vials, 10 ng/tube
EK1282-DA Human IL18BP Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)093.7187.5375750150030006000
O.D.0.0540.1060.1620.2640.6030.8361.3182.059

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-18BP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12292421271179372003
Standard deviation7.0747.12110.68.8965.59110.16
CV (%)5.8%5.5%5.2%7.6%7%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL18BP in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11221281421391328.16.1%
Sample 292488590786889621.272.3%
Sample 321272341246922952308122.415.3%
*number of samples for each test n=16.

Gene/Protein Information For IL18BP (Source: Uniprot.Org, NCBI)

Gene Name

IL18BP

Full Name

Interleukin-18-binding protein

Weight

21.099kDa

Alternative Names

IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa IL18BP FVHa, IL18BP interleukin 18 binding protein interleukin-18-binding protein|MC51L-53L-54L homolog gene product|tadekinig-alfa

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL18BP, check out the IL18BP Infographic

IL18BP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL18BP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Human IL-18BP ELISA Kit PicoKine®

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL18BP in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

B. Tiwari

Verified customer

Asked: 2019-06-21

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL18BP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-06-21

Question

Q: how do I analyze ELISA data? I have performed IL18BP level in serum.

Verified Customer

Verified customer

Asked: 2018-12-22

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-12-22

Question

Q: What is the optimal O.D. value for IL18BP ELISA kit? I used your IL18BP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL18BP even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-10-31

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL18BP ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL18BP that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-10-31

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL18BP using the EK1282 Human IL18BP Picokine® ELISA Kit?

R. Varma

Verified customer

Asked: 2018-10-05

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-10-05

Question

Q: if the enzyme conjugated IL18BP antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL18BP antigen?

Verified Customer

Verified customer

Asked: 2018-03-26

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-03-26

Question

Q: how to thaw whole blood sample for IL18BP ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-03-26

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL18BP for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-03-26

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

D. Dhawan

Verified customer

Asked: 2016-07-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-07-24

Question

Q: is it okay to use citrate plasma as samples in Human IL18BP Picokine® ELISA Kit (Catalog # EK1282)?

L. Dhar

Verified customer

Asked: 2014-06-07

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL18BP causing disruption of its protein structure. IL18BP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL18BP ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2014-06-07

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