Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-2 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC. Cited in 2 publication(s).
Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Info At A Glance
|Size:||For 5 plates, 96 wells each, reagents only, plates are not included|
|Sample Type:||cell culture supernates, serum and plasma( heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
|Product Name||Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all IL-2 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-2 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.|
|Cite This Product||Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0397)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma( heparin, EDTA, citrate).|
|Immunogen/Standard||Expression system for standard: E.coli, Immunogen sequence: A21-T153|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||For 5 plates, 96 wells each, reagents only, plates are not included|
Gene/Protein Basic Information For IL2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3558|
|Species Of This Entry||Human|
|Alternative Names||IL-2|Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF|
|Post Tranlational Modifications||Phosphorylation; Acetylation; Methylation; Cleavage; Glycosylation; Reduction; Dephosphorylation; Demethylation; Biotinylation; Oxidation; Ribosylation; Nitration; Deacetylation; Ubiquitination|
|Gene Location||4q27, on Chromasome 4, gene sequence: NC_000004.12 (122449479..122456725, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL2 (Source: Uniprot.org, NCBI)
|Protein Function||Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.|
|Interacting Proteins||IL2RA; IL2RB|
|Related Diseases||Neoplasms; Malignant Neoplasms; Leukemia; Autoimmune Reaction; Carcinoma; Inflammation; Lymphoma; Melanoma; Autoimmune Diseases; Immunologic Deficiency Syndromes; Decreased Immunologic Activity [pe]; Malignant Paraganglionic Neoplasm; Infective Disorder; Neoplasm Metastasis; Hiv Infections; Diabetes Mellitus; Diabetes Mellitus, Insulin-dependent; Renal Cell Carcinoma; Kidney Neoplasm; Arthritis|
|Related Pathways||Immune Response; Secretion; Cell Activation; Cell Proliferation; T Cell Activation; Pathogenesis; Cell Growth; Cytokine Production; T Cell Proliferation; Lymphocyte Proliferation; Cell Cycle; Lymphocyte Activation; Cell Differentiation; Cell Death; Hypersensitivity; Localization; B Cell Differentiation; Cell Development; Aging; Inflammatory Response|
|Background||Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.|
|Scientific References||PMID: 6312994 by Maeda S., et al. Cloning of interleukin 2 mRNAs from human tonsils.|
PMID: 6403867 by Taniguchi T., et al. Structure and expression of a cloned cDNA for human interleukin-2.
Kit Components And Assay QC Data Details Of Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EZ0397-CAP||96-well plate precoated with anti-Human IL-2 antibody||1|
|EZ0397-ST||lyophilized recombinant Human IL-2 standard||10ng/tube|
|EZ0397-DA||biotinylated anti-Human IL-2 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EZ0397 Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 2
|A novel derivative of (-) mycousnine produced by the endophytic fungus Mycosphaerella nawae, exhibits high and selective immunosuppressive activity on T %u2026||PubMed ID 28725220|
|il-8 regulates the stemness properties of cancer stem cells in the small-cell lung cancer cell line h446||PubMed ID 30254465|
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