Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-3 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Info At A Glance
|Size:||For 5 plates, 96 wells each, reagents only, plates are not included|
|Sample Type:||cell culture supernates, serum and plasma( heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
|Product Name||Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all IL-3 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-3 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.|
|Cite This Product||Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0402)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma( heparin, EDTA, citrate).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: A20-F152|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||For 5 plates, 96 wells each, reagents only, plates are not included|
Gene/Protein Basic Information For IL3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3562|
|Species Of This Entry||Human|
|Alternative Names||IL-3|Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Methylation; Dephosphorylation; Acetylation; Oxidation; Reduction; Hydroxylation; Cleavage; Prenylation; Ribosylation|
|Gene Location||5q31.1, on Chromasome 5, gene sequence: NC_000005.10 (132060655..132063204)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL3 (Source: Uniprot.org, NCBI)
|Protein Function||Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.|
|Tissue Specificity||Activated T-cells, mast cells, natural killer cells.|
|Related Diseases||Leukemia; Myeloid Leukemia; Leukemia, Myelocytic, Acute; Neoplasms; Malignant Neoplasms; Malignant Paraganglionic Neoplasm; Inflammation; Immunologic Deficiency Syndromes; Diphtheria; Myeloid Leukemia, Chronic; Tissue Adhesions; Lymphoma; Anemia; Cell Transformation, Neoplastic; Asthma; Autoimmune Diseases; Myeloproliferative Disease; Autoimmune Reaction; Infective Disorder; Dysmyelopoietic Syndromes|
|Related Pathways||Cell Proliferation; Cell Growth; Pathogenesis; Cell Cycle; Cell Death; Secretion; Cell Activation; Immune Response; Cell Development; Cytokine Production; T Cell Activation; Cell Differentiation; Programmed Cell Death; Localization; S Phase; T Cell Proliferation; Angiogenesis; Cell Adhesion; Glycosylation; Hemopoiesis|
|Background||The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.|
|Scientific References||PMID: 3497843 by Dorssers L., et al. Characterization of a human multilineage-colony-stimulating factor cDNA clone identified by a conserved noncoding sequence in mouse interleukin-3.|
PMID: 3127463 by Otsuka T., et al. Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones.
Kit Components And Assay QC Data Details Of Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EZ0402-CAP||96-well plate precoated with anti-Human IL-3 antibody||1|
|EZ0402-ST||lyophilized recombinant Human IL-3 standard||10ng/tube|
|EZ0402-DA||biotinylated anti-Human IL-3 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EZ0402 Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
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