Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-32 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Info At A Glance
|Size:||For 5 plates, 96 wells each|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA).|
|Sample Volume:||100μl per well|
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
|Product Name||Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all IL32 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-32 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.|
|Cite This Product||Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1599)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: E.coli, A31-K234|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||For 5 plates, 96 wells each|
Gene/Protein Basic Information For IL32 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||9235|
|Species Of This Entry||Human|
|Alternative Names||IL32|IL32; IL-32; IL-32alpha; IL-32beta; interleukin 32; interleukin-32 theta; interleukin-32; natural killer cell transcript 4; Natural killer cells protein 4; NK4; NK4IL-32delta; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; TAIFIL-32gamma; Tumor necrosis factor alpha-inducing factor|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Biotinylation; Methylation; Demethylation; Acetylation|
|Gene Location||16p13.3, on Chromasome 16, gene sequence: NC_000016.10 (3065403..3069530)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL32 (Source: Uniprot.org, NCBI)
|Protein Function||Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF- alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.|
|Tissue Specificity||Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells.|
|Interacting Proteins||CEBPA; PRKCD; PRTN3|
|Related Diseases||Inflammation; Arthritis; Rheumatoid Arthritis; Malignant Neoplasms; Inflammatory Bowel Diseases; Autoimmune Reaction; Neoplasms; Crohn Disease; Tissue Adhesions; Autoimmune Diseases; Influenza; Tuberculosis; Virus Diseases; Intestinal Diseases; Lung Diseases; Colitis; Vasculitis; Leukemia; Ulcer; Lung Diseases, Obstructive|
|Related Pathways||Immune Response; Pathogenesis; Secretion; Inflammatory Response; Cytokine Production; Cell Death; Cell Adhesion; Cell Cycle; Cell Growth; Cell Proliferation; Localization; Cell Activation; Cell Migration; Adaptive Immune Response; Cell Cycle Arrest; Interleukin-32 Production; Innate Immune Response; Methylation; Angiogenesis; Viral Replication|
|Background||Interleukin 32 (Il32) is a protein that in humans is encoded by the IL32 gene. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.|
|Scientific References||PMID: 1729377 by Dahl C.A., et al. Identification of a novel gene expressed in activated natural killer cells and T cells.|
PMID: 15664165 by Kim S.-H., et al. Interleukin-32: a cytokine and inducer of TNFalpha.
Kit Components And Assay QC Data Details Of Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EZ1599-CAP||96-well plate precoated with anti-Human IL32 antibody||1|
|EZ1599-ST||lyophilized recombinant Human IL32 standard||Catalog number|Description|Quantity|Buffers EZ1599-CA|Rabbit anti- human IL-32 polyclonal antibody(Capture Antibody)|500 μL, 0.4mg/mL(recommended dilution 1:100)|0.5% Na2HPO4, 0.02% Sodium azide and 50% Glycerol EZ1599-DA|Biotinylated goat anti- human IL-32 polyclonal antibody(Detection Antibody)|500 μL, 1.4μg/mL(recommended dilution 1:100)|0.02% Sodium azide, 0.5%BSA, 0.2%Tris, 1%NaCl and 30% Glycerol EZ1599-ST|Lyophilized recombinant human IL-32 standard|10ng/tubex2| AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|500ul(recommended dilution 1:100)||
|EZ1599-DA||biotinylated anti-Human IL32 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EZ1599 Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-20minConcentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.003|0.071|0.130|0.214|0.399|0.825|1.421|2.055
We measured random samples of Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|84|757|1872|92|823|2035 Standard deviation|3.61|46.18|102.96|4.32|51.03|107.86 CV(%)|4.3%|6.1%|5.5%|4.7%|6.2%|5.3%
We measured the performance consistency of Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
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