Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-33 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Info At A Glance
|Size:||For 5 plates, 96 wells each|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.|
|Sample Volume:||100μl per well|
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
|Product Name||Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all IL-33 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-33 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.|
|Cite This Product||Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0929)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.|
|Immunogen/Standard||Expression system for standard: E.coli, S112-T270|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||For 5 plates, 96 wells each|
Gene/Protein Basic Information For IL33 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||90865|
|Species Of This Entry||Human|
|Alternative Names||IL-33|C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Methylation; Biotinylation; Reduction; Citrullination|
|Subcellular Localization||Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).|
|Gene Location||9p24.1, on Chromasome 9, gene sequence: NC_000009.12 (6214591..6257983)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL33 (Source: Uniprot.org, NCBI)
|Protein Function||Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).|
|Tissue Specificity||Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.|
|Interacting Proteins||HIST1H2AG; IL1RL1|
|Related Diseases||Inflammation; Asthma; Arthritis; Rheumatoid Arthritis; Autoimmune Reaction; Eosinophilia; Ulcer; Atherosclerosis; Fibrosis; Colitis; Rhinorrhea; Ulcerative Colitis; Autoimmune Diseases; Dermatitis; Allergy; Dermatitis, Atopic; Allergic Asthma; Hypertrophy; Myocardial Infarction; Tissue Adhesions|
|Related Pathways||Pathogenesis; Secretion; Immune Response; Cytokine Production; Inflammatory Response; Angiogenesis; Cell Activation; Localization; Mast Cell Activation; Cell Differentiation; Basophil Activation; Wound Healing; Cell Adhesion; Cell Proliferation; Endothelial Cell Activation; Anaphylaxis; Proteolysis; Hypersensitivity; Protein Secretion; Reverse Transcription|
|Background||Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.|
|Scientific References||PMID: 12819012 by Baekkevold E.S., et al. Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules.|
PMID: 10566975 by Onda H., et al. Identification of genes differentially expressed in canine vasospastic cerebral arteries after subarachnoid hemorrhage.
Kit Components And Assay QC Data Details Of Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EZ0929-CAP||96-well plate precoated with anti-Human IL-33 antibody||1|
|EZ0929-ST||lyophilized recombinant Human IL-33 standard||Catalog number|Description|Quantity|Buffers EZ0929-CA|Rabbit anti- human IL-33 polyclonal antibody(Capture Antibody)|500 μL, 0.4mg/mL(recommended dilution 1:100)|0.5% Na2HPO4, 0.02% Sodium azide and 50% Glycerol EZ0929-DA|Biotinylated goat anti- human IL-33 polyclonal antibody(Detection Antibody)|500 μL, 3.5μg/mL(recommended dilution 1:100)|0.02% Sodium azide, 0.5%BSA, 0.2%Tris, 1%NaCl and 30% Glycerol EZ0929-ST|Lyophilized recombinant human IL-33 standard|10ng/tubex2| AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|500ul(recommended dilution 1:100)||
|EZ0929-DA||biotinylated anti-Human IL-33 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EZ0929 Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 25-30minConcentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.029|0.057|0.101|0.189|0.335|0.632|1.152|2.098
We measured random samples of Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|30|260|484|28|282|511 Standard deviation|1.74|16.38|37.75|1.76|22.27|45.99 CV(%)|5.8%|6.3%|7.8%|6.3%|7.9%|9%
We measured the performance consistency of Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
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