|Pack Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.|
Data & Images
|Product Name||Human IL-33 PicoKine™ ELISA Kit|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-33. 96wells/kit, with removable strips.|
|Cite This Product||Human IL-33 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0929)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Immunogen||Expression system for standard: E.coli; Immunogen sequence: S112-T270|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Capture Antibody||monoclonal antibody from mouse|
|Detection Antibody||polyclonal antibody from goat|
|Natural Levels||Some research articles suggesting the natural levels of IL33 are listed below:|
|Storage||Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|EK0929-CAP||96-well plate precoated with anti-Human IL33 antibody||1|
|EK0929-ST||lyophilized recombinant Human IL33 standard||10ng/tubex2|
|EK0929-DA||biotinylated anti-Human IL33 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
|AR0030-E||PBS washing buffer||Powder for 1000ml|
Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.See Prices For Extra Components
Material Required But Not Provided
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||30759 MW|
|Protein Function||Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528). .|
|Tissue Specificity||Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. .|
|Subcellular Localization||Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent.|
|Alternative Names||Interleukin-33;IL-33;Interleukin-1 family member 11;IL-1F11;Nuclear factor from high endothelial venules;NF-HEV;Interleukin-33 (95-270);Interleukin-33 (99-270);Interleukin-33 (109-270);IL33;C9orf26, IL1F11, NFHEV;|
|Research Areas|||cardiovascular|angiogenesis|cytokines| immunology|innate immunity|interleukins| cardiovascular|heart|hypertrophy||
Background for Interleukin-33
Human IL-33 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Typical Data Obtained from Human IL-33 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 25-30min)
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.