Human IL-37/IL-1F7 ELISA Kit PicoKine®

IL-37/IL-1F7 ELISA kit for Human

Human IL-37/IL1F7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL37 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 4 publication(s).

Product Info Summary

SKU: EK1363
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human IL-37/IL-1F7 ELISA Kit PicoKine®

View all IL-37/IL-1F7 ELISA kits

SKU/Catalog Number

EK1363

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-37/IL1F7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL37 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-37/IL-1F7 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1363)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V46-D218

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1363 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1363 is reactive to IL37 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1363 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-37/IL-1F7

Interleukin 37 (IL-37) also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. And it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. And IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 can emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.

Kit Components

Catalog Number Description Quantity
EK1363-CAP Anti-Human IL37 Pre-coated 96-well strip microplate 1
EK1363-ST Human IL37 Standard 2 vials, 10 ng/tube
EK1363-DA Human IL37 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0130.0590.0920.1630.3280.5641.0531.880

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-37/IL-1F7, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..

Some articles we found to cite concentrations of IL-37/IL-1F7 in samples: 23697556 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-37/IL-1F7 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3414544036134419
Standard deviation2.615.2422.882.957.122.62
CV (%)7.7%4.1%5.2%8.2%5.3%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-37/IL-1F7 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 134343329322.066.4%
Sample 21451391481591477.254.9%
Sample 34404504484314427.491.6%
*number of samples for each test n=16.

Gene/Protein Information For IL37 (Source: Uniprot.Org, NCBI)

Gene Name

IL37

Full Name

Interleukin-37

Weight

24.126kDa

Superfamily

IL-1 family

Alternative Names

FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein IL37 FIL1, FIL1(ZETA), FIL1Z, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-23, IL-37, IL1F7, IL1H4, IL1RP1 interleukin 37 interleukin-37|FIL1 zeta|IL-1 zeta|IL-1F7b (IL-1H4, IL-1H, IL-1RP1)|IL-1X protein|IL1F7 (canonical product IL-1F7b)|interleukin 1 family member 7|interleukin 1, zeta|interleukin-1 homolog 4|interleukin-1 superfamily z|interleukin-1-related protein|interleukin-23

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL37, check out the IL37 Infographic

IL37 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL37: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1363 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Profiling of B-Cell Factors and Their Decoy Receptors in Rheumatoid Arthritis: Association With Clinical Features and Treatment Outcomes

Profiling of Canonical and Non-Traditional Cytokine Levels in Interferon-β-Treated Relapsing–Remitting-Multiple Sclerosis Patients

Profiling of Canonical and Non-Traditional Cytokine Levels in Interferon-?-Treated Relapsing?Remitting-Multiple Sclerosis Patients

Yu J, Lu Y, Li Y, Xiao L, Xing Y, Li Y, Wu L. J Pharm Pharmacol. 2015 Sep;67(9):1240-50. Doi: 10.1111/Jphp.12415. Epub 2015 Apr 16. Role Of S100A1 In Hypoxia-Induced Inflammatory Response In Cardiomyocytes Via Tlr4/Ros/Nf-??b Pathway.

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14 Customer Q&As for Human IL-37/IL-1F7 ELISA Kit PicoKine®

Question

Can I use PBS of other concentrations (such as 0.0067M or 1X) as the wash buffer except 0.01M for Human IL-37/IL-1F7 ELISA Kit PicoKine® (EK1363)?

Verified customer

Asked: 2022-03-25

Answer

Our lab hasn't tested PBS of other concentrations and we are uncertain if they can be used. We suggest to prepare the wash buffer by using the provided recipe. Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. Preparation of 0.01M PBS: Add 8.5g Nacl, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

Boster Scientific Support

Answered: 2022-03-27

Question

In your Datasheet it is not mentioned if the kit recognizes all isoforms (A to E), or only certain isoforms. If the kit recognizes every isoform, is there any difference in the antibodies' affinity? Are the epitopes/immunogen sequences of the capture and detection antibodies, conserved among all the isoforms?

Verified customer

Asked: 2020-08-26

Answer

Our lab hasn't experimentally validated if the kit is able to recognize all isoforms. The immunogen sequence of both the capture and detection antibodies in EK1363 is Lys27-Asp192 Accession # Q9NZH6-2. According to the sequence of the antibody pairs, the kit is specific to isoform A.

Boster Scientific Support

Answered: 2020-08-26

Question

Q: What is the optimal O.D. value for IL-37 ELISA kit? I performed your IL-37 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-37 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-06-17

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-37 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-37 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-06-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-01-06

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-09-18

Question

Why can the EK1363 standard only be stored for 48 hours at -20 °C after being dissolved?

Verified customer

Asked: 2019-09-10

Answer

For the Human IL-37/IL1F7 ELISA Kit PicoKine™ (EK1363), the protein would have the risk of degrading if the standard is stored longer than 48 hours at -20 °C after being dissolved.

Boster Scientific Support

Answered: 2019-09-11

Question

Does the dissolved standard have a long-term storage method?

Verified customer

Asked: 2019-09-10

Answer

Long-term storage of dissolved standard is not recommended to be used. Please use fresh dissolved standard.

Boster Scientific Support

Answered: 2019-09-11

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IL-37 using the EK1363 Human IL-37 Picokine® ELISA Kit?

Y. Wright

Verified customer

Asked: 2019-02-01

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-02-01

Question

We have a customer who bought your Human IL-37/IL1F7 ELISA Kit KOA0824. However, she has some questions now and hope to get your help on the followings: Could you please tell the customer why the IL-37/IL1F7 standard can only be stored for 48 hours at -20

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

The protein would have the risk of degrading if the standard is stored longer than 48 hours at -20

Boster Scientific Support

Answered: 2018-12-14

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IL-37 in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

J. Moore

Verified customer

Asked: 2018-10-18

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-37 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-10-18

Question

Q: can I use citrate plasma as samples in Human IL-37 Picokine® ELISA Kit (Catalog # EK1363)?

Verified Customer

Verified customer

Asked: 2018-08-05

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-37 causing degradation of its protein structure. IL-37 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-37 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-08-05

Question

Q: Can IL-37 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

E. Martinez

Verified customer

Asked: 2018-07-02

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-07-02

Question

Q: if the enzyme conjugated IL-37 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-37 antigen?

Verified Customer

Verified customer

Asked: 2018-05-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-05-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-11-02

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-11-02

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