Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-5 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Info At A Glance
|Size:||For 5 plates, 96 wells each, reagents only, plates are not included|
|Sample Type:||cell culture supernates, serum, plasma (heparin, EDTA) and urine.|
|Sample Volume:||100μl per well|
|Product Name||Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all IL-5 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects IL-5 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.|
|Cite This Product||Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0407)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma (heparin, EDTA) and urine.|
|Immunogen/Standard||Expression system for standard: sf21; Immunogen sequence: I20-S134|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||For 5 plates, 96 wells each, reagents only, plates are not included|
Gene/Protein Basic Information For IL5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3567|
|Species Of This Entry||Human|
|Alternative Names||IL-5|BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I|
|Post Tranlational Modifications||Phosphorylation; Acetylation; Glycosylation; Biotinylation; Methylation; Carboxylation; Phosphorothioation; Cleavage; Citrullination; Deglycosylation|
|Gene Location||5q31.1, on Chromasome 5, gene sequence: NC_000005.10 (132539194..132556864, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL5 (Source: Uniprot.org, NCBI)
|Protein Function||Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.|
|Interacting Proteins||CSF2RB; IL5RA|
|Related Diseases||Asthma; Inflammation; Eosinophilia; Allergy; Malignant Neoplasms; Neoplasms; Infective Disorder; Leukemia; Rhinorrhea; Autoimmune Reaction; Allergic Rhinitis (disorder); Dermatitis; Lymphoma; Hay Fever; Autoimmune Diseases; Arthritis; Lung Diseases; Nasal Polyps; Allergic Asthma; Dermatitis, Atopic|
|Related Pathways||Pathogenesis; Secretion; Cytokine Production; Immune Response; Cell Proliferation; Cytokine Secretion; Cell Activation; Cell Differentiation; Sensitization; Hypersensitivity; Transport; Chemotaxis; Eosinophil Activation; T Cell Activation; Reverse Transcription; Cell Cycle; Eosinophil Migration; Eosinophil Differentiation; Cell Growth; B Cell Differentiation|
|Background||Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.|
|Scientific References||PMID: 3024129 by Azuma C., et al. Cloning of cDNA for human T-cell replacing factor (interleukin-5) and comparison with the murine homologue.|
PMID: 2824500 by Tanabe T., et al. Molecular cloning and structure of the human interleukin-5 gene.
Kit Components And Assay QC Data Details Of Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EZ0407-CAP||96-well plate precoated with anti-Human IL-5 antibody||1|
|EZ0407-ST||lyophilized recombinant Human IL-5 standard||10ng/tube|
|EZ0407-DA||biotinylated anti-Human IL-5 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EZ0407 Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of Human IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
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